The center line represents the sample median, the box limits consist of the 25th and 75th percentiles, the whiskers span 1.5x the interquartile range, and each RNA-seq sample is represented by a point on the graph (n = 4 per group). d, Representative Western Blot images for primary...
Neutrophils are implicated in multiple homeostatic and pathological processes, but whether functional diversity requires discrete neutrophil subsets is not known. Here, we apply single-cell RNA sequencing to neutrophils from normal and inflamed mouse tissues. Whereas conventional clustering yields multiple alt...
Skip to main contentSkip to article Journals & Books Help Search My accountSign in View PDF
COPD patients were matched to healthy non-smoker control donors ± 5 years of age wherever possible (total number of participants 18 aged between 20-70, mean age range 41-50, Gender M 8: F 9). Negative magnetic selection (STEMCELL Technologies, Vancouver) was carried out to further purify ...
Neutrophils were isolated from both the blood and bronchoalveolar lavage fluid of 21 patients with CF (12 male, 9 female; mean age 7.5 years, range 0.25-16.4 years) and 17 non-CF subjects (9 male, 8 female; mean age 5.4 years, range 0.2-13.1 years). The ex vivo phagocytic rate of ...
[35]. Peripheral blood was obtained from heparinized venous blood of normal voluntary donors. Aliquots (100 μL) of whole blood were then incubated in the presence or absence of samples with different concentrations at 37°C for 30 min. Thereafter, cells were stimulated with LPS (0.25 μg/...
Alterations in haematological variables reported in this study were found to be within the normal physiological range for calves of this age, and thus no negative effects on animal welfare were determined using these measures. Furthermore, the difference in rectal body temperature between supplemented ...
They also reported that neutrophils were activated by fMLP in the range from 1 nM to 1 mM peptide, with 100 nM fMLP producing a maximal response [44]. After being activated by 100 nM fMLP, normal human neutrophils have a remarkable degree of depolarization, and the peak membrane ...
Wellcome Sanger Institute, Wellcome Genome Campus, Cambridge, UK Eddie Cano-Gamez, Katie L. Burnham & Emma E. Davenport Kennedy Institute of Rheumatology, University of Oxford, Oxford, UK Yasemin-Xiomara Zurke, Claudia Monaco & Irina A. Udalova ...
In response to exposure to a variety of stimuli derived either from pathogens (e.g. formyl methionyl-leucyl-phenylalanine) or from the host (a range of cytokines and growth factors), the neutrophil is able to rapidly mobilise these granule contents to the plasma membrane. Coupled with this, ...