cell suspension was passed through 70- and 40-μm cell strainers. After RBC lysis, cells were washed and resuspended in staining buffer (1 × PBS/2% FBS). Total and trypan blue-negative (viable) cells were counted with an automated cell counter (BioRad). Representative images of propidium...
consecutive days during which the patient shows an absolute neutrophil count higher than 0.5 × 109/l, along with sustained platelet (>20 × 109/l) and hemoglobin levels (>80 g/l), and does not require transfusion.4After the engraftment, the donor-derived immune system undergoes...
In step716, the enumerated EGC are reported. The enumerated EGC can be reported as an absolute count and/or as a percentage of the total white blood cell count. The enumerated EGC can also be reported as a percentage and/or fraction of any other cell population, such as, for example, ...
4 PCR amplification was performed in an automated thermal cycler (Bio Phase; Bio Excellence, Colchester, UK) using denaturation for 2 minutes, primer annealing at 55°C, and extension at 72°C for 2 minutes (30 cycles). Twenty microliters of PCR products was fractionated by agarose gel ...
Here, we report a haptotaxis assay, based on the classic under-agarose assay, which combines an optical patterning technique to generate surface-bound formyl peptide gradients as well as an automated imaging and analysis of a large number of migration trajectories. We show that human neutrophils ...
(α-CR1 and α-CR3). While CR3-blocking antibodies did not affect phagocytosis of C3b-E. coli, we observed that CR1-blocking antibodies potently reduced phagocytosis (Fig.1E). Inhibiting CR1 reduced both the absolute number of bacteria associated with neutrophils (Fig.1E) as well as the ...
[Ca2+]I, applying the Ca2+ionophore ionomycin to samples after progesterone stimulation produced the same absolute rise in peak [Ca2+]iin the presence and absence of 75 µM Alb (Supplementary Fig.1,d, e, f). Furthermore, Alb was shown to have no direct effect on CatSper currents ...