Neutrophil-based drug delivery system possesses excellent advantages in targeting at tumour because neutrophils are easily recruited by chemotactic factor in tumor microenvironment. Herein, we developed a novel tactic of multistage neutrophils-based nano
1× 106 human neutrophils were lysed in 200 μL ice-cold H2O and boiled for 10 min at 95 degrees; lysates were centrifuged at 18,000 g at 4°C for 10 min to remove cell debris and snap frozen. Glycogen content was measure using a fluorimetric assay (Sigma-Aldrich). Extracellular Acidif...
Activated neutrophils (ANs) are known to release reactive oxygen species that may cause oxidative damage to surrounding tissues. We determined if ANs could induce lipid peroxidation (LP) in human red cells and investigated the mechanism involved in this interaction. We studied neonatal glucose-6-...
10,000 termed (CP-10), has potent chemotactic activity for murine and human myeloid cells. We examined the ability of a synthetic CP-10 hinge region peptide CP-10(42-55) and rCP-10 to act as ch... JM Devery,NJC King,CL Geczy - 《Journal of Immunology》 被引量: 153发表: 1994年...
What is a dangerously low neutrophil count? In general, an adult who has fewer than 1,000 neutrophils in a microliter of blood has neutropenia. If the neutrophil count is very low,fewer than 500 neutrophils in a microliter of blood, it is called severe neutropenia. ...
Here, as part of the Immunological Genome (ImmGen) Project, we employed droplet-based single-cell RNA-seq (scRNA-seq) to profile more than 17,000 mouse neutrophils from bone marrow, blood and spleen as well as from blood, joint, lung and peritoneum from animals undergoing experimental sterile...
Differentiated PLB-985 cells or human neutrophils (200,000 cells/100 µl) or NETs (generated from 200,000 cells/100 µl) were added to purified fibrinogen or plasma. Normalized percentage of MaxOD (compared to thrombin only controls) was quantified. Other concentrations: thrombin (0.1 U/...
After 5 min on ice, the cell lysates were centrifuged at 10,000 g for 10 min at 4 °C. After centrifugation, cellular lysates (30 µg) were also subjected to 12% SDS-PAGE, followed by western blot with antibodies against JAK, phospho-STATs, and STATs. Western blots were visualized ...
9,10.重要なことに、このような血管新生TANはNAMPTの活性の上昇を示し、好中球の腫瘍偏光にその重要な役割を示唆している。 Ly6G抗体を用いた好中球の枯渇またはそれらの移行の阻害(CXCR2抗体)は、腫瘍血管新生、増殖、および転移1、8の減少をもたらす。それにもかかわらず、生成されたモノクロー...
Prior work in mice has demonstrated that after this initial early infiltration, neutrophil tissue levels decline significantly but still persist at lower levels in the post-MI subacute period (∼7,000 cells/heart at 14 d post-MI) [10,39]. In our study, we observed ∼15,000 neutrophils/...