Here, using a microfluidic approach, we analyzed the migration of neutrophil-like HL-60 cells to a traveling wave of the chemoattractants N-formyl-methionyl-leucyl-phenylalanine (fMLP) and leukotriene B4 (LTB4). We found that under a pulsatile wave that travels at a speed of 95 and 170 m/...
First, we found that MWCNTs decreased the viability of neutrophil-like differentiated HL-60 cells but not undifferentiated HL-60 cells. Because neutrophil-like differentiated HL-60 cells exhibit enhanced phagocytic activity, the cytotoxicity of MWCNTs is dependent on the intracellularly localized MWCNTs....
Here, we examined the mitochondrial morphology of neutrophil-like differentiated HL-60cells after chemoattractant N-formyl-Met-Leu-Phe (fMLP) stimulation. We found that mitochondrial morphology changes to a tubular form after fMLP stimulation. Mitochondrial OXPHOS activity and mitochondrial complex II ...
The human leukemia cell line (HL-60) is an alternative to primary neutrophils in research studies. However, because HL-60 cells proliferate in an incompletely differentiated state, they must undergo differentiation before they acquire the functional prop
that the expression of CD11b mRNA was up-regulated comparing with the negative control groups since DMSO 24 hours(P0.01).All data suggest that neutrophil-like cells can be derived from HL-60 cells induced by DMSO,and neutrophil-like cells could be the substitution model for neutrophil research...
The effects of granulocyte colony-stimulating factor (G-CSF) on development of O−2generating system during differentiation of HL-60 cells to neutrophil-like cells have been studied. G-CSF enhanced O−2generating ability of HL-60 cells whose differentiation had been initiated by dimethylsulfoxide...
Consistent with public domain RNA expression profiles17,18, we have confirmed that ZBTB11 protein is highly expressed in human Jurkat (T cells), K562 cells (a BCR-ABL positive blast crisis erythroleukaemia) and HL60 (promyelocytic leukaemia) cells (Fig. 2f). Lower expression in HepG2 liver ...
Hosoi, Saccharomyces cerevisiae and Candida albicans stimulate cytokine secretion from human neutrophil- like HL-60 cells differentiated with retinoic acid or dimethylsulfoxide, Bio- sci. Biotechnol. Biochem. 73 (2009) 2600e2608.Saegusa, S., Totsuka, M., Kaminogawa, S., Hosoi, T., 2009. ...
Differentiated HL60 cells (dHL60) are a widely-used model of human neutrophils for migration and chemotaxis14. In the current study, we show for the first time that SSL11 disrupts neutrophil motility by induction of cell adhesion. These findings provide a new therapeutic target against S. ...
The cells were diluted 1:1 with media containing 50 μM cytochrome c (Sigma), with or without 0.1 μM fMLF (Sigma), with or without 60 U/mL superoxide dismutase (SOD; Sigma). The cells were incubated at 37°C for 15 minutes. The cells were then chilled on ice, centrifuged, and ...