Neutrophil-like cells stained positive for acid phosphatase and Sudan black, expressed MPO, and produced a dose-dependent respiratory burst response after stimulation with phorbol esters (PMA) and calcium ionophore A23187 (CaI). Treatment of neutrophil-like cultures with PMA, CaI or zymosan induced ...
First, we found that MWCNTs decreased the viability of neutrophil-like differentiated HL-60 cells but not undifferentiated HL-60 cells. Because neutrophil-like differentiated HL-60 cells exhibit enhanced phagocytic activity, the cytotoxicity of MWCNTs is dependent on the intracellularly localized MWCNTs....
The human leukemia cell line (HL-60) is an alternative to primary neutrophils in research studies. However, because HL-60 cells proliferate in an incompletely differentiated state, they must undergo differentiation before they acquire the functional prop
These results suggest that MFN2 is involved in chemotaxis of differentiated HL-60cells depending on mitochondria.doi:10.1016/j.bbrc.2019.04.037Mazaki, YuichiTakada, ShingoNio-Kobayashi, JunkoMaekawa, SatoshiHigashi, TsunehitoOnodera, YasuhitoSabe, Hisataka...
that the expression of CD11b mRNA was up-regulated comparing with the negative control groups since DMSO 24 hours(P0.01).All data suggest that neutrophil-like cells can be derived from HL-60 cells induced by DMSO,and neutrophil-like cells could be the substitution model for neutrophil research...
Thus, in recent years it has become evident that different metabolic routes determine the fate of immune cells and impact the inflammatory response. Future studies are needed to validate the source of monocyte subsets and how key metabolic adaptations directly support their identity and function. In...
There are comments on PubPeer for publication: CD13 Neutrophil-like myeloid-derived suppressor cells exert immune suppression through Arginase 1 expression in pancreatic ductal adenocarcinoma (2017)
Dendritic cells: translating innate to adaptive immunity. Curr Top Microbiol Immunol. 2006;311:17–58. PubMed CAS Google Scholar Lee RM, White MR, Hartshorn KL. Influenza a viruses upregulate neutrophil toll-like receptor 2 expression and function. Scand J Immunol. 2006;63(2):81–9. ...
The effects of granulocyte colony-stimulating factor (G-CSF) on development of O−2generating system during differentiation of HL-60 cells to neutrophil-like cells have been studied. G-CSF enhanced O−2generating ability of HL-60 cells whose differentiation had been initiated by dimethylsulfoxide...
Here we show Staphylococcal Superantigen-Like protein 11 (SSL11) from MRSA USA300_FPR3757 mediated differentiated human neutrophil-like cells (dHL60) motility arrest by inducing cell adhesion and “locking” cells in adhesion stage, without inducing oxidative burst. Pre-incubation of SSL11 with ...