The different steps involved in phagocytosis and bacterial killing are : chemotaxis, opsonisation, surface, contact, ingestion and digestion. The specific membrane receptors (for the Fc fragment of IgG and for the third component of the complement) and extra-cellular factors are involved in ...
Major neutrophil functions include phagocytosis, degranulation, and NET release (Fig. 1), all of which have been associated with the antimicrobial functions of the cell. Neutrophils eliminate both intra- and extracellular pathogens through phagocytosis, which is the process of engulfing and digesting fo...
Under steady-state conditions, human and mouse neutrophils have a half-life of 6–12 h in the bloodstream before migrating to the spleen, lymph nodes, liver, and bone marrow. Neutrophils remain for 1–2 days to perform phagocytosis and cytotoxic functions, ultimately undergoing spontaneous apoptosi...
Neutrophil phagocytosis and neutrophil phagosomal acidification are two critical steps in this process [26]. Both can be assessed by flow analysis of neutrophils phagocytosing bioparticles coupled to pH-sensitive and pH- insensitive dyes [27]. Neutrophil subsets, including CD16dim/CD62Lbright cells...
In dialysis patients, neutrophil phagocytosis was significantly decreased to 76% of normal levels. Only a small percentage of neutrophils did not show any phagocytic activity at all. The percentage of inactive neutrophils was around 1% in healthy controls and significantly increased to 2.6% and 2.2%...
There are at least three forms of endocytosis that could allow internalization of NPs by neutrophils: phagocytosis, clathrin-mediated endocytosis (CME), and macropinocytosis. 6.1. Phagocytic pathway The phagocytosis process occurs primarily in professional phagocyte cells, and is the major mechanism of ...
Phagocytosis-independent antimicrobial activity of mast cells by means of extracellular trap formation. Blood. 2008;111:3070–80. Article CAS Google Scholar Yousefi S, Morshed M, Amini P, Stojkov D, Simon D, von Gunten S, et al. Basophils exhibit antibacterial activity through extracellular ...
B Neutrophils of HC and HD donors were incubated with cytochalasin D to halt phagocytosis. The extracellular bacterial killing (NETosis only) was performed as in A. To show that the extracellular bacterial killing derives from NETosis, the same experiment was conducted ...
Fig. 3: Linking a fPep to vancomycin enhances the phagocytosis activity of neutrophils. aThe infection-on-a-chip device used to monitor neutrophil migration and phagocytosis over time present in a six-well plate. Within this microfluidic device the egg-like microchambers in the main channel were...
functions, through production of active oxygen, secretion of degradative infiltration enzymes, release of cytokines and phagocytosis. The infiltration of neutrophils is not limited to the case of invasion of foreign bodies, such as viruses and bacteria. At the time myocardial infarction or an organ ...