Cell subtypeMarkerLocalizationSpecies Pan-granulocytesCD11bSurfaceHuman and mouse CD13SurfaceHuman CD15SurfaceHuman CD16/32SurfaceMouse CD32SurfaceHuman CD33SurfaceHuman NeutrophilsElastaseSecretedHuman and mous
Identification of CD Marker Expression and Neutrophil Surface Marker Changes in Health and Disease using High-throughput Screening Flow CytometryNeutrophil hyperactivation can contribute to tissue damage in inflammatory diseases. Although many cell-surface proteins are known to be expressed on neutrophils ...
Its expression on neutrophil cell surface, how- ever, increases substantially within a few minutes after the cell comes into contact with bacteria or endotoxins (29, 30). This unique property enables CD11b to be used as a potential early warning marker for detection of bacterial infection. ...
Unfortunately, unlike in preclinical animal models, no unique cell surface marker allows rapid and transient depletion of neutrophils; therein lies the benefit of a particle-based approach to neutrophil blocking in thromboinflammation. Thus, extensive work has explored using polymeric particles and other...
Although neutrophils can be visually identified based on the shape of their nuclei and cytoplasmic granularity, they can also be identified byflow cytometry. Mouse neutrophils are commonly identified based on the cell surface expression of Ly-6G and CD11b/Integrin alpha M. ...
Each stage has a unique phenotype that is comprised of both morphologic features and cell surface markers. In the first step, the cell transitions into the myeloblast stage. The promyelocyte stage is characterized by the appearance of azurophilic granules. Specific granules start to develop at ...
Neutrophils express a large number of cell surface receptors for the recognition of microbial invasion. Some of those receptors are capable of innate recognition of microbial structures while others (such as Fc-receptors) are linked to the activation of the adaptive immune response, and yet other ...
Nevertheless, neutrophil activation is impaired with aging; the reason lies within defects in downstream signaling pathways of cell-surface receptors such as the mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase (PI-3K), protein kinase B (PKB), janus kinase –signal transducer and ...
The presence of alloantibodies due to previous pregnancy or transfusion can obscure this picture. A neutrophil autoantibody or cell-bound immune complexes may be the cause of this phenomenon, which may be a useful marker of Felty's syndrome in patients with rheumatoid arthritis....
Cell surface marker staining and flow cytometry The isolated single cells from primary tumor, lung, PB or BM were washed with cell staining buffer for twice and then resuspend with 100 μl of cell staining buffer (Biolegend, #420201). Then fluorochrome-conjugated anti-mouse monoclonal antibod...