According to the irreversible catabolic character of the non-phosphorylating reaction, the GapN-complemented clone was unable to grow on gluconeogenic substrates, This metabolic engineering approach demonstrates that a pure catabolic Embden-Meyerhof pathway with no net energy yield is feasible. (C) 1999...
Due to its simplified presentation and easier interpretability, the reduced network allows the user to quickly find fluxes through metabolites and reaction modes or pathways of interest. In this manuscript, we first demonstrate NetRed on a simple network consisting of glycolysis and the pentose ...
BioNetSim: A Petri net-based modeling tool for simulations of biochemical processes. Protein and Cell 3:225-229. (2012). J Gao,L Li,X Wu,... - 蛋白质与细胞:英文版 被引量: 0发表: 2012年 Biochemical Reaction Pathway Analysis Using Petri Net A systematic procedure has been developed to ...
Quantitative polymerase chain reaction To assess Piezo1 expression, RNA was extracted using the RNeasy Mini Kit (QIAGEN). The purity of RNA was then assessed using a NanoDrop (Thermo Fisher Scientific), and complementary DNA (cDNA) was synthesized with the SuperScript IV VILO Master Mix kit (In...
Only once the capacitation is completed the spermatozoa can respond to the oocyte interaction with the exocytosis of acrosome content, acrosome reaction (AR). These post-ejaculatory events are under the attention of Researchers from more than fifty years but their basic knowledge is still ...
While there is some theoretical support for a rate-affinity trade-off for particular reaction schemes12,13, this depends on untestable assumptions about the free energy profile and it does not apply to typical reaction schemes of membrane transport processes, such as facilitated diffusion. We will...
using this product. Note: Niacin may cause temporary flushing, tingling, skin reddening, and sensations of heat, especially if taken on an empty stomach. This is a natural and harmless reaction. Do not use if either tamper-evident seal is broken or missing. Keep out of the reach of ...
of the Golgi-localized AE2a anion exchanger. In the acidic Golgi lumen, imported bicarbonate anions and protons then facilitate a common buffering reaction that yields carbon dioxide and water before their egress back to the cytoplasm via diffusion or water channels. The flattened morphology of the...
(D) The value of the X intercept is equal to Vmax, (E) The value of the Y-intercept is equal to Vmax. 30. Which of the following statements concerning the reaction catalyzed by ATCase is true? (A) One ATP molecule is used in the ATCase reaction, (B) CTP is a feedback activat...
When a Petri net is a model of a biological system, transitions are counterparts of active components of the system; they represent some elementary processes occurring in the system (e.g., chemical reaction), while places are counterparts of passive components (e.g., chemical compounds). Arcs...