The cell pellet was resuspended in 50 mL of ice-cold lysis buffer consisting of 150 mM NaCl, 20 mM Tris pH 8.0, 5 mM βME, 0.8 μM aprotinin, 2 μg/mL leupeptin, 2 μM pepstatin A, 1 mM PMSF and 20 μM DNQX. Cells were lysed by sonication using a Misonix sonicator with ...