T7E1protocol 48~72h 3.TargetsitePCR. Mostimportantstep! RobustandcleanPCRamplificationiscritical.Thus, nestedPCR(notrequiredbutrecommended)isuseful. WeusePrimer3programforprimerdesign (http://frodo.wi.mit.edu/primer3/) **1 st PCR:600~1kbsizearoundTargetsite ...
T7E1说明书(NEB)
T7E1说明书(NEB).pdf,T7E1 protocol 1. CRISPR Transfection. 48~72h 2. Genomic DNA prep. 3. Target site PCR. 4. Denature/Reannealing 5. T7 endonuclease I reaction. 6. Agarose gel eletrophoresis T7E1 protocol 1. CRISPR Transfection. Methods and conditio
T7E1说明书(NEB)
(T7E1) according to the recommended protocol. Lane 1: NEB PCR Marker (NEB #N3234), Lane 2: untreated genomic DNA, Lane 3: untreated genomic DNA digested with T7E1, Lane 4: DNA transfected with Cas9 and guide RNA, Lane 5: DNA transfected with Cas9 and guide RNA and digested with T7E1...
TE说明书NEB
35 T7E1 支 4 E3321 36 dNTP Solution Mix 支 8 N0447L 37 T7 DNA ligase 支 2 M0318L 38 T4 DNA 连接酶 支 5 M0202L 39 O-Glycosidase & Neuraminidase Bundle 支 4 E0540S 40 α2-3,6,8 Neuraminidase 支 3 P0720S 41 Rapid? PNGase F 支 6 P0710S 42 Gel Loading Dye, Purple (6X...
(T7E1) according to the recommended protocol. Lane 1: NEB PCR Marker (NEB #N3234), Lane 2: untreated genomic DNA, Lane 3: untreated genomic DNA digested with T7E1, Lane 4: DNA transfected with Cas9 and guide RNA, Lane 5: DNA transfected with Cas9 and guide RNA and digested with T7E1...
T7E1说明书(NEB).pdf,T7E1 protocol 1. CRISPR Transfection. 48~72h 2. Genomic DNA prep. 3. Target site PCR. 4. Denature/Reannealing 5. T7 endonuclease I reaction. 6. Agarose gel eletrophoresis T7E1 protocol 1. CRISPR Transfection. Methods and conditio