T7E1说明书(NEB)
T7E1protocol 48~72h 3.TargetsitePCR. Mostimportantstep! RobustandcleanPCRamplificationiscritical.Thus, nestedPCR(notrequiredbutrecommended)isuseful. WeusePrimer3programforprimerdesign (http://frodo.wi.mit.edu/primer3/) **1 st PCR:600~1kbsizearoundTargetsite ...
T7E1说明书(NEB).pdf,T7E1 protocol 1. CRISPR Transfection. 48~72h 2. Genomic DNA prep. 3. Target site PCR. 4. Denature/Reannealing 5. T7 endonuclease I reaction. 6. Agarose gel eletrophoresis T7E1 protocol 1. CRISPR Transfection. Methods and conditio
TE说明书NEB
(T7E1) according to the recommended protocol. Lane 1: NEB PCR Marker (NEB #N3234), Lane 2: untreated genomic DNA, Lane 3: untreated genomic DNA digested with T7E1, Lane 4: DNA transfected with Cas9 and guide RNA, Lane 5: DNA transfected with Cas9 and guide RNA and digested with T7E1...
(T7E1) according to the recommended protocol. Lane 1: NEB PCR Marker (NEB #N3234), Lane 2: untreated genomic DNA, Lane 3: untreated genomic DNA digested with T7E1, Lane 4: DNA transfected with Cas9 and guide RNA, Lane 5: DNA transfected with Cas9 and guide RNA and digested with T7E1...
T7E1说明书(NEB).pdf,T7E1 protocol 1. CRISPR Transfection. 48~72h 2. Genomic DNA prep. 3. Target site PCR. 4. Denature/Reannealing 5. T7 endonuclease I reaction. 6. Agarose gel eletrophoresis T7E1 protocol 1. CRISPR Transfection. Methods and conditio