T7E1protocol 48~72h 3.TargetsitePCR. Mostimportantstep! RobustandcleanPCRamplificationiscritical.Thus, nestedPCR(notrequiredbutrecommended)isuseful. WeusePrimer3programforprimerdesign (http://frodo.wi.mit.edu/primer3/) **1 st PCR:600~1kbsizearoundTargetsite ...
28 PvuI-HF(星选酶) 支 2 R3150L 29 Golden Gate Assembly Kit (BsmBI-v2) 支 2 E1602L 30 Golden Gate BsaI-HF? v2 支 3 E1601S 31 Q5? High-Fidelity 2X Master Mix 支 6 M0492L 32 Exonuclease VIII 支 2 M0545S 33 ATP 支 2 P0756S 34 MmeI 支 4 R0637S 35 T7E1 支 4 E3321 3...