Choose a DNA, RNA, qPCR calculator from NEB, a leader in production and supply of reagents for the life science industry.
Use the NEB Tm Calculator to estimate an appropriate annealing temperature when using NEB PCR products.
NEBioCalculator(nebiocalculator)是由New England Biolabs(NEB)开发的一款专注于生物医学研究的在线计算工具,广泛应用于分子生物学和遗传学等领域。它提供了多种实用功能模块,帮助科研人员快速完成实验设计中的复杂计算,包括基础计算工具和进阶实验支持。 主要功能模块 NEBioCalculator的主要功能模块可以分为两大类:基础计...
NEBioCalculator® FAQs & 问题解决指南 FAQs What are some potential problems with the ligation reaction using T4 DNA Ligase that can lead to transformation failure? What are some other problems that should be considered when trouble shooting a transformation problem?
NEBioCalculator® REBASE® FAQs & 问题解决指南 FAQs Do degenerate recognition sites need to be palindromic? I tested your restriction enzyme on the substrate DNA recommended by NEB, and it appears to be active, however it does not digest my DNA. What could be the reason?
Quick Tips - Do I need a 5' phosphate for ligation? NEBioCalculator® - Using the Ligation module Quick Tips - Electroporation after ligation PrevNext其它感兴趣的产品 Hi-T4™ 耐热 DNA 连接酶 快速末端平齐化™ 试剂盒 NEB® 5-alpha E. coli 感受态细胞(高效级) 平末端/TA 连接酶预...
NEBioCalculator® REBASE® FAQs & 问题解决指南 FAQs How can this enzyme be inactivated? Do I have to set-up digests with Time-Saver™ qualified enzymes for 5-15 minutes? Can I digest longer? Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)? Is this enzym...
工具& 资源 选择指南 Isoelectric Points (pI) for Restriction Enzymes NEB Diluent and Buffer Table Type IIS Restriction Enzymes Web 工具 Double Digest Finder Enzyme Finder NEBcutter™ v3.0 NEBioCalculator® REBASE®FAQs & 问题解决指南FAQs...
Phusion 对退火温度的要求与大多数 PCR 酶不同。请访问 www.neb.cn/tmcalculator,帮助您确定最佳的 Phusion 退火温度。引用& 技术文献 引用文献 产品引用文献查找工具 专题文章 Polymerase Fidelity: What is it, and what does it mean for your PCR?质控...
Read Coverage Calculator 此工具可以轻松计算与 NGS 协议中的读取覆盖相关的值。 Tm 计算器在设计 PCR 反应方案时使用此工具以提供帮助确定扩增子的最佳退火温度。只需输入您的 DNA 聚合酶即可、引物浓度和引物序列以及 Tm 计算器将指导您获得成功的反应条件。数据库 ...