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此外,也可选择 NEB Quick Ligation 快速连接试剂盒(#M2200S)[30 次反应] 或(#M2200L)[150 次反应]),在室温条件下反应 5 分钟,即可完成 DNA 平末端和粘性末端的连接。 参考文献 Engler, M.J. and Richardson, C.C. (1982). P.D. Boyer(Ed.), 5, 3. San Diego: Academic Press....
NEBioCalculator® - Using the Ligation module NEBioCalculator® - Using the ds: mass < — > moles module to plan an NEBuilder® HiFi DNA Assembly Reaction NEBioCalculator® - Using the ds: mass -> ends module to plan a phosphorylation reaction using T4 Polynucleotide Kinase (T4 PNK)...
NEBUILDER® Assembly Tool 2.0 Fragments Amplified by PCR NEBUILDER® Assembly Tool 2.0 Restriction Enzyme Digest NEBioCalculator® - Using the Ligation module NEBioCalculator® - Using the ds: mass < — > moles module to plan an NEBuilder® HiFi DNA Assembly ReactionPassion...
V. Potapov et al., Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synth Biol 7, 2665–2674 (2018). 产品类别: DNA Ligases, DNA 组装、克隆和突变试剂盒产品 应用: High-throughput cloning and automation solutions, NEBridge...
NEBioCalculator® FAQs & 问题解决指南 FAQs Do I need to transform the Instant Sticky-end Ligase Master Mix reactions immediately? Can the ligation reaction produced by the Instant Sticky-end Ligase Master Mix be directly used to transform electrocompetent cells?
NEBioCalculator® FAQs & 问题解决指南 FAQs What are some potential problems with the ligation reaction using T4 DNA Ligase that can lead to transformation failure? What are some other problems that should be considered when trouble shooting a transformation problem?
Cloning Ligation, NEBridge® Golden Gate Assembly 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 M0202V-20 T4 DNA LigaseM0202VVIAL-201 x 0.025 ml400,000 units/ml T4 DNA Ligase Reaction BufferB0202SVIAL-201 x 1 ml10 X ...
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look for Time-Saver and HF enzymes for the ultimate in convenience. Use Tm Calculator to calculate optimum annealing temperature for PCR primers when using NEB polymerases and buffers. Use NEBioCalculator to convert DNA or RNA mass to moles, calculate insert ligation amounts, convert OD to conc...