Total RNA in cells was extracted usingTrizolreagent (Invitrogen, Carlsbad, CA, USA) at 48 h after transfection. Thereverse transcriptionreaction was performed using a First-Strand cDNA Synthesis kit (Takara Bio, Tokyo, Japan) with 1 μg of RNA in a final volume of 20 μl. The newly synth...
This alloy caused expansion in the volume of the samples with continuous penetration of the molten zinc along the cobalt layer. Hence, the gaps between WC particles got widened, which formed a cake structure [36]. The relation between the quantity of fine WC recycled powder and the ratio of...