Total RNA in cells was extracted usingTrizolreagent (Invitrogen, Carlsbad, CA, USA) at 48 h after transfection. Thereverse transcriptionreaction was performed using a First-Strand cDNA Synthesis kit (Takara Bio, Tokyo, Japan) with 1 μg of RNA in a final volume of 20 μl. The newly synth...
1. The milling process was carried out using SPEX CertiPrep 8000-series for milling time of 2 h and the ball to powder weight ratio of 5:1 and ceramic balls with diameter of 5 mm. The vial is made from stainless steel that has an internal volume of 50 ml. The milling ...