Following recovery, the water consumption of the mice was restricted to 1.2 ml per day, to train them in the behavioural task. Following training, a small craniotomy for acute recording was made at 0.5 mm anterior and 1.7 mm lateral relative to bregma in the left hemisphere. A ...
The mobile-phase solvents consisted of 0.1% formic acid, 99.9% water (solvent A) and 0.1% formic acid, and 99.9% acetonitrile (solvent B). The peptides were gradient-eluted at a flow rate of 350 nl min−1 using a 180 min gradient. A Q Exactive Plus mass spectrometer (Thermo...
A reagent solution of 48 mL MOPS buffer (10 mM, pH 7.0, 47 mL), 1 mL DCPIP (20 mg dissolved in 5 mL of DI water), and 1 mL phenazine methosulfate (PMS) (45 mg dissolved in 5 mL of DI water) were prepared. Analytical samples (~3 uL) were mixed with ...
The identity of the spring modules was confirmed via MALDI-TOF mass spectrometry (Bruker). 350 µg lyophilized peptide was dissolved in PBS at a final concentration of 5 mg/ml, mixed with Alexa Fluor 555 maleimide (Thermo Fisher Scientific) at a 1.5-fold molar surplus and then ...
additional sources encompassed mostly bottled and spring water. In the longer term, elites’ strategies to cope with reduced public water availability extended to the development of private water sources, such as rainwater harvesting systems or boreholes located on the premises of their households. Fi...
water vapor at 24 °C (10 mL min−1,pH2O = 2.9 kPa).cH2evolution rate of various cocatalysts loaded SrTiO3:Al irradiated with 370 nm LED (5.1 mW cm−2) under saturated water vapor balanced with Ar at 24 °C (10 mL min−1,pH2O = 2.9...
For high predation simulation treatments, we constructed exclusion cages of nylon mesh (1 cm mesh size) attached to a PVC frame (the nylon mesh we used did not significantly affect physical factors such as light or water flow in previous work where we quantified wave attenuation in high pred...
Before administration, we incubated the tamoxifen solution in a 37 °C water bath for 5 min. To induce the nuclear translocation of CreERT2 and conditional knockout of the candidate gene, we intraperitoneally injected the tamoxifen solution (100 µl daily) for 4 consecutive days. We ...
MDCK cells were trypsinized to a single cell suspension at 4 × 104 cells/ml in complete medium containing 2% Matrigel (BD Biosciences). Cell-medium-Matrigel suspensions (350 µl) were plated into 35 mm dish with gridded coverslips (MatTek Corporation, P35G-1.5-14-CGRD), pre-coated...
Cells were dehydrated in the TES buffer for 30 min before rapidly re-hydrated by the addition of 40 mL pre-cooled water into the mixture. Periplasmic extraction was recovered by centrifugation at 20,000 g for 30 min. The supernatant was added with 150 mM NaCl, 2 mM MgCl2, ...