Over the past two decades, more than 80 metropolitan cities across the world have faced severe water shortages due to droughts and unsustainable water use. Future projections are even more alarming, since urban water crises are expected to escalate and most heavily affect those who are socially, ...
Following recovery, the water consumption of the mice was restricted to 1.2 ml per day, to train them in the behavioural task. Following training, a small craniotomy for acute recording was made at 0.5 mm anterior and 1.7 mm lateral relative to bregma in the left hemisphere. A ...
Before administration, we incubated the tamoxifen solution in a 37 °C water bath for 5 min. To induce the nuclear translocation of CreERT2 and conditional knockout of the candidate gene, we intraperitoneally injected the tamoxifen solution (100 µl daily) for 4 consecutive days. We ...
The identity of the spring modules was confirmed via MALDI-TOF mass spectrometry (Bruker). 350 µg lyophilized peptide was dissolved in PBS at a final concentration of 5 mg/ml, mixed with Alexa Fluor 555 maleimide (Thermo Fisher Scientific) at a 1.5-fold molar surplus and then ...
Mice had free access to food and water except during the starvation period before glucose or insulin tolerance testing. Aging mice were fed a normal diet (PicoLab Rodent Diet 20, LabDiet), mice on the HFD experiments were fed an HFD (TD.06414, 60% of kcal from fat; Envigo). Flow ...
water vapor at 24 °C (10 mL min−1,pH2O = 2.9 kPa).cH2evolution rate of various cocatalysts loaded SrTiO3:Al irradiated with 370 nm LED (5.1 mW cm−2) under saturated water vapor balanced with Ar at 24 °C (10 mL min−1,pH2O = 2.9...
A reagent solution of 48 mL MOPS buffer (10 mM, pH 7.0, 47 mL), 1 mL DCPIP (20 mg dissolved in 5 mL of DI water), and 1 mL phenazine methosulfate (PMS) (45 mg dissolved in 5 mL of DI water) were prepared. Analytical samples (~3 uL) were mixed with ...
Cells were dehydrated in the TES buffer for 30 min before rapidly re-hydrated by the addition of 40 mL pre-cooled water into the mixture. Periplasmic extraction was recovered by centrifugation at 20,000 g for 30 min. The supernatant was added with 150 mM NaCl, 2 mM MgCl2, ...
Then, they were dissociated in PBS containing 1.5 mg/mL collagenase type ǁ and performed digestion by continually shaking in a 37 °C water bath shaker at 100–200 rpm. The digestion procedure was repeated 7–8 times for 5 min each time, and the supernatant was collected into ...
Freeze-dried soil samples (1 g for each) were vortexed at maximum speed in 10 mL of acetonitrile: isopropanol: water (volume ratio 1:1:1) for 1 min and ultrasonic oscillation for 30 min at 4 °C. The mixture was centrifuged at 13000 x g at 4 °C for 15 min. The...