One liter YP medium containing 2% glucose (v/v) was inoculated with an overnight preculture. Cells were grown to OD600 1 at 26 °C, followed by 1 h heat shock at 38 °C. Cells were harvested by centrifugation (4800 × g for 10 min at 4 °C). Pellets were washed...
When cell density reached 2.5 × 106 cells/milliliter, the cells were transiently transfected with the pcDNA3.4-based expression plasmids using PEI MAX (Polysciences, USA). The cells were harvested 96 h after transfection for protein purification. All of the primers were listed in ...
4.According to the passage, Lake Como of Italy ___. A.is home to many famous people B.is the largest lake in Europe C.began to attract tourists from last century D.looks the most beautiful during spring A 解析:细节理解题。根据第四个湖:Today, many celebrities(名人) have houses...
3D bioprinting of tissues and organs will find application in tissue engineering, research, drug discovery and toxicology. Additive manufacturing, otherwise known as three-dimensional (3D) printing, is driving major innovations in many areas, such as eng
Protein backbone atoms were constrained using a spring force (in form of k(∆x)2)83 and movement of hydrogen atoms were constrained by SHAKE algorithm86. The systems were then simulated for 5 ns in a NPT ensemble with a pressure of 1 bar applied using Berendsen barostat87 and ...
For soil samples, 500 g of soil was introduced into 2 liters of an inorganic salt solution (K2HPO4 0.02 g L˗1, MgSO4·7H2O 0.02 g L-1, KNO3 0.02 g L -1, pH adjusted to 7.0) and then stirred using a magnetic stirrer for 2 hours. For AS samples, ...
S. & Spring, D. R. Diversity-oriented synthesis: producing chemical tools for dissecting biology. Chem. Soc. Rev. 41, 4444–4456 (2012). Article CAS PubMed Google Scholar Maurya, S. K. & Rana, R. An eco-compatible strategy for the diversity-oriented synthesis of macrocycles exploiting ...
Two liters of each seawater sample (GR1 and GR3) were filtered through a 0.2-μm pore-size polyethersulfone membrane filter (Supor, Pall). DNA was extracted directly from the membrane filters using DNeasy PowerWater Kit (Qiagen) according to the manufacturer’s instructions. The V4-V5 regions...
Processing bodies (p-bodies) are a prototypical phase-separated RNA-containing granule. Their abundance is highly dynamic and has been linked to translation. Yet, the molecular mechanisms responsible for coordinate control of the two processes are unclea
Molecular Cloning: A Laboratory Manual 2nd edn, (Cold Spring Harbor Laboratory Press, 1989). Onsager, L. The effects of shape on the interaction of colloidal particles. Ann. N.Y. Acad. Sci. 51, 627–659 (1949). Article CAS Google Scholar Adams, M.E., Dogic, Z., Keller, S.L. ...