Mass analyses were carried out on a Thermo Scientific Q-Exactive HRM (ESI source, 3.5 kV ionization voltage, 300 °C capillary temp., 55 arb sheath gas, aux gas flow rate at 5 L/min, S-lens RF level 50) coupled with Accela Open AS 1250. Samples (5 µL) were desalted ...
We then tested whether expression ofALMT1andSTOP1is regulated by Pi availability. One hour after transfer from the +Pi to −Pi medium the expression level ofALMT1increases and reaches a plateau at 24 h (Fig. 1e). However, on both mediaALMT1expression is abrogated in strongstop1mutants...
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