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"Compact RNA editors with natural miniature Cas13j nucleases." Nature Chemical Biology.(20240919,浙江大学,姚远,马斌,陈学新,李果)通过对土壤微生物大规模宏基因组数据进行智能挖掘,发现一个可高效靶向切割RNA的极小型Cas13j蛋白家族,蛋白大小为...
Base editors(碱基编辑器) Double-strand breaks(双链断裂) Cytidine-to-guanine base editors(胞嘧啶到鸟嘌呤碱基编辑器) 研究背景 碱基编辑器(BEs)无需双链DNA切割就能引入碱基替换。除了精确替换,BEs还会通过不明机制产生低频的'随机'副产物。 C到G碱基编辑器(CGBEs)产生的中间双链断裂(DSBs)在多种副产物的形...
Nature Cell Biology26,294–304 (2024)Cite this article 7884Accesses 21Altmetric Metrics Abstract Base editors (BEs) introduce base substitutions without double-strand DNA cleavage. Besides precise substitutions, BEs generate low-frequency ‘stochastic’ byproducts through unclear mechanisms. Here, we perf...
目前,不依赖DNA双链断裂和模板参与的单碱基编辑器(base editors)是治疗遗传病强有力的基因编辑工具。现有的碱基编辑技术CBEs(C-to-T),CGBEs(C-to-G), GBEs(C-to-G/C-to-A)和ABEs(A-to-G)可实现胞嘧啶转换/颠换编辑以及腺嘌呤的转换突变。由于缺少切除肌苷的内源DNA糖苷酶,ABEs产生的A-to-G产物纯度...
近一年来，中国科学院神经研究所杨辉团队连续取得突破，在Cell， Protein & Cell，Nature Communications ，Nature Cell Biology，Nature Protocol，Nature Methods 等杂志上发表了7项重要研究成果，在基因编辑领域取得重大进展，iNature系统总结了这些研究成果：【1】微生物和病毒之间的竞争性共同进化导致CRISPR-Cas防御传染...
2023年4月,中国科学院遗传与发育生物学研究所高彩霞团队在Nature Biotechnolgy期刊发表了题为:Precise integration of large DNA sequences in plant genomes using PrimeRoot editors的研究论文。该研究将团队之前开发的ePPE(Engineered Plant Prime Editor)与刘如谦团队开发的epegRNA(Engineered pegRNA)结合,在植物细胞内...
4. Zhang, X. et al. Increasing the efficiency and targeting range of cytidine base editors through fusion of a single-stranded DNA-binding protein domain.Nature cell biology22, 740-750 (2020). 5. Chen, L. et al. ...
2023年4月,高彩霞研究员团队在Nature Biotechnolgy期刊发表了题为Precise integration of large DNA sequences in plant genomes using PrimeRoot editors的研究论文。 该研究将团队之前开发的ePPE(Engineered Plant Prime Editor)与美国博德研究所刘如谦团队开发的epegRNA(Engineered pegRNA)结合,在植物细胞内建立了dual-eP...