(Cat# 123110, 1.0 µg per million cells in 100 µL volume), PerCP/Cyanine5.5 anti-mouse/human CD11b Antibody [M1-70] (Cat# 101227, 0.25 µg per million cells in 100 µL volume) and Ultra-LEAF™ Purified anti-mouse CD8a Antibody [53-6.7] (Cat# 100764) were ...
(KAPA Library Quantification Kits For Roche 454 GS Titanium platform, Cat. No: KK4821 and BioRad CFX96 Touch™ Real-Time PCR Detection System; C1000 Thermal cycler) according to the manufacturer’s instructions with samples diluted 1:500, 1:1000, and 1:2000 in 10 mM Tris-HCl, pH ...
Chu S-A, Tsai P-W, Pan J-S (2006) Cat swarm optimization. Lecture notes in computer science (including subseries Lecture notes in artificial intelligence and lecture notes in bioinformatics), LNAI, vol 4099, pp 854–858. cited by (since 1996) 8 Iordache S (2010) Consultant-guided search...
Tetum and the other national languages shall be valued and developed by the StateFootnote 1 Section 159 (working languages) Indonesian and English shall be working languages within civil service side by side with official languages as long as deemed necessary. http://timor-leste.gov.tl/?cat=...
1; see present location in Fig. 6), are dominantly underlain by Archaean continental crust, and formed part of the ancient nucleus of Madagascar and India. Conversely, the Seychelles (and probably also the Laxmi Ridge) is likely underlain by Palaeoproterozoic rocks39, as in northern Madagascar...
and 4% of known reptile species are identified in cat diets. 97% of species consumed are <5 kg in adult body mass, though much larger species are also eaten. The species accumulation curves are not asymptotic, indicating that our estimates are conservative. Our results demonstrate that cats...
Fig. 1:Pum1/2CKOsuppresses the development of colorectal cancer. a,cRT-qPCR examination of the relativePum1(a) orPum2(c) mRNA levels in human CRC specimens from 22 patients as compared to adjacent normal tissues. Actin was used as an internal control.b,dmRNA expression ofPum1(b) orPum2...
Alamar blue (Thermo Fisher Scientific, Cat# DAL1025) was added and cells were incubated at 37 °C in the dark for 4 h. Fluorescence was measured on the Spectramax spectrophotometer and was normalized to the DMSO control. Flow cytometry Cell preparation and harvesting Glioma cells grown on...
One arm of the experiment was pre-treated with 100 nM GSI (Abcam cat. no. ab145891) for 30 min before EGTA treatment. The pre-treated GSI arm and another non-pre-treated arm were then washed with PBS and incubated for 10 min in PBS and 4 mM EGTA for 10 min at 37 ...
For cholesterol biosynthesis inhibition, 10-week-old Prkcif/fPrkczf/f;Villin-Cre mice were treated with a combination of oral atorvastatin (Selleckchem Cat# S5715; 50 mg/kg) and intraperitoneal dipyridamole (Selleckchem Cat# S1895; 120 mg/kg) daily for 4 weeks. Control mice were ...