An oligonucleotide containing a CPD (AflII CPD, HPLC-purified; TriLink Biotechnology) was synthesised and stored in 10 mM Tris-Hcl (pH 8.0), 1 mM EDTA at −20 °C. To introduce the oligo into the plasmid of interest (pGC504), 4 × 200 µg of the relevant plasmid was ...
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Melbourne Protein Characterisation Platform, Bio21 Molecular Science and Biotechnology Institute, Melbourne, VIC, Australia Yee-Foong Mok Division of Rheumatology, Inflammation, and Immunity, Brigham and Women’s Hospital, Harvard Medical School, Boston, MA, USA Josephine F. Reijneveld, D. Branch Mood...