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(clone C9B7W, rat IgG1,BioLegend, 1:100), anti-CD44 in BV 570 (clone IM7, rat IgG2b, BioLegend,1:100), anti-CD73 in BV 605 (clone TY/11.8, rat IgG1, BioLegend, 1:100),anti-CD25 in BV 650 (clone PC61, rat IgG1, BioLegend, 1:100), anti-PD-1 in BV785 (clone 29...
(clone C9B7W, rat IgG1,BioLegend, 1:100), anti-CD44 in BV 570 (clone IM7, rat IgG2b, BioLegend,1:100), anti-CD73 in BV 605 (clone TY/11.8, rat IgG1, BioLegend, 1:100),anti-CD25 in BV 650 (clone PC61, rat IgG1, BioLegend, 1:100), anti-PD-1 in BV785 (clone 29...
(30% OptiPrep密度梯度介质、134 mM蔗糖、5 mM CaCl2、3 mM Mg 0.04% IGEPAL CA-630和0.17 U L1重组RNase抑制剂)置于300 l 40% OptiPrep溶液(40% OptiPrep密度梯度培养基、96 mM蔗糖、5 mM CaCl2、3 mM Mg(CH3COO)2、10 mM Tris HCl pH 7.8、0.1 mM EDTA pH 8.0、1mMβ-巯基乙醇、0.03% IGEPAL...
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