After washes with PBS with 0.1% Tween-20 (PBST), cells were incubated with anti-mouse minus and anti-rabbit plus PLA probes (Sigma-Aldrich) at 37 °C for 1 h. Following the manufacturer’s instructions, the PLA reaction was performed with the Duolink In Situ Detection Reagents. Cells...
the single-cell signature associated with XO4+microglia to previously described mouse microglia gene signatures in AD (Supplementary Fig.6a–e) revealed a substantial number of XO4+-specific DEGs, which are enriched uniquely for inflammatory-related processes including response to interferon-gamma, T ...
the single-cell signature associated with XO4+microglia to previously described mouse microglia gene signatures in AD (Supplementary Fig.6a–e) revealed a substantial number of XO4+-specific DEGs, which are enriched uniquely for inflammatory-related processes including response to interferon-gamma, T ...
The second of these is a fine new book, an excellent field guide for the "odes" of eastern North America, published by Princeton University Press in 2011. Another book, with some quite interesting text, is "Natural History of Delmarva Dragonflies and Damselflies", essays of a lifelong observ...
A major informatic challenge in single cell RNA-sequencing analysis is the precise annotation of datasets where cells exhibit complex multilayered identities or transitory states. Here, we present devCellPy a highly accurate and precise machine learning-
Circular RNAs (circRNAs) are a regulatory RNA class. While cancer-driving functions have been identified for single circRNAs, how they modulate gene expression in cancer is not well understood. We investigate circRNA expression in the pediatric malignanc
Modelling the interactions that arise from neural dynamics in seizure genesis is challenging but important in the effort to improve the success of epilepsy surgery. Dynamical network models developed from physiological evidence offer insights into rapidl
(ER81050, Epicenter), followed by A-addition using Klenow fragment (3’ to 5’ exo minus) and dATP. The Y-shaped adapters were then ligated to DNA ends using T4 DNA ligase. Libraries were amplified with Illumina primers for 16 cycles and library fragments from 150 bp to 300 bp ...