Novel psychoactive substances (NPS) represent a broad class of drugs new to the illicit market that often allow passing drug-screening tests. They are characterized by a variety of structures, rapid transience on the drug scene and mostly unknown metabol
R.P.B. is supported by grant funding through the Canadian Institutes of Health Research and the Natural Sciences and Engineering Research Council of Canada and holds a Canada Research Chair in Brain Lipid Metabolism. R.J.S.L. holds an Ontario Graduate Scholarship and receives funding support ...
All chemical and reagents were of analytical grade. Purified water was daily obtained with a Milli-Q system from Millipore (Bedford, MA, USA). Glacial acetic acid (HAC), acetone, acetonitrile (ACN), ethylene glycol (EG), methanol (MeOH), formic acid, glycerol, and ethanol (EtOH) were ac...
The large impact of infectious disease in swine on animal welfare and productivity has increased interest in selection for pigs that are less susceptible to pathogens and that are more disease resilient, which has been defined as the ability to maintain relatively undiminished performance levels when ...
Kijanska, M.; Kelm, J. In Vitro 3D Spheroids and Microtissues: ATP-Based Cell Viability and Toxicity Assays. InAssay Guidance Manual [Internet]; Eli Lilly& Company and the National Center for Advancing Translational Sciences: Bethesda, MD, USA, 2016; pp. 1–13. [Google Scholar] ...
2024, 29, 30. [Google Scholar] [CrossRef] Pierantonelli, I.; Svegliati-Baroni, G. Nonalcoholic Fatty Liver Disease: Basic Pathogenetic Mechanisms in the Progression From NAFLD to NASH. Transplantation 2019, 103, e1–e13. [Google Scholar] [CrossRef] Loguercio, C.; De Girolamo, V.; de...
肌动蛋白细胞骨架磷酸化目的:研究ATP缺失时大鼠近端肾小管上皮细胞(NRK52E)肌动蛋白磷酸化水平的改变.方法:建立细胞的体外ATP缺失模型,用免疫印迹技术检测细胞内骨架组分及胞浆组分中肌动蛋白的分布变化;用二维电泳法分离并比较细胞内磷酸化和非磷酸化的肌动蛋白含量的改变;用免疫共沉淀法及免疫印...
研究了胞外ATP对胞内Ca2+信号的影响,初步探讨了其作用机制.实验表明:胞外ATP能够分别使大鼠胰腺β细胞和INS-1细胞内的游离Ca2+浓度显著升高,但2种细胞的钙信号来源不同.在大鼠胰腺β细胞中,胞外ATP主要通过动员胞内钙库释放而引起胞浆内Ca2+浓度显著增高;而在INS-1细胞内,胞外ATP主要通过引起胞外Ca2+内流而...
In normal brains, expression of endothelial VEGFR-2 was found to be low or undetectable; however, the levels of VEGFR-2 vessels and the expression of endothelial VEGFR-2 elevate with tumor grade, more in GBM [36]. Additionally, in another study VEGFR-2 expression mainly depends on the ...
9 Molecular Diagnostics and Personalized Therapeutics Unit, University of Ha’il, Hail 55476, Saudi Arabia 10 Department of Medical Laboratory Sciences, King Abdulaziz University, P.O. Box 80216, Jeddah 21589, Saudi Arabia addShow full affiliation list ...