结合高脂(HFD-fed)饲喂LDLR ko小鼠,可以诱导以肥胖、高胆固醇血症为特征的代谢综合征、高甘油三酯血症和胰岛素抵抗症状;类似于人NASH模型为特征的模型[4]。 图2. 肝纤维化,A.健康人肝脏切片HE染色,B. NASH病人肝脏切片HE染色,C.LDLR ko小鼠肝脏切片HE染色,D高脂饲料饲喂LDLR ko小鼠诱导NASH的肝脏切片HE染色。
二、模型构建方法 NASH模型主要有膳食动物模型【高脂饮食(high-fat diet, HFD)模型、蛋氨酸和胆碱缺乏(methionine-choline deficient, MCD)饮食模型、高胆固醇和高胆酸盐(high-cholesterol and high-cholate, HCh Ch)模型】、化学诱导模型【四氯化碳(CCl4)诱发模型、乙硫氨酸诱发模型、高糖高脂饮食+链脲佐菌素(STZ)诱...
plantarum-mediated NASH regulation in a mouse model to understand its therapeutic effect. We used a choline-deficient high-fat diet (CD-HFD)-induced murine model that recapitulated the critical features of human metabolic syndrome and investigated the effect of L. plantarum on...
278 In an MCD-induced mouse NASH model, vitamin E supplementation reduced hepatic inflammation and fibrosis by reducing the expression of the proapoptotic BCL2-related X (BAX), TGF-dead, cyclooxygenase-2 (COX-2), and matrix metalloproteinase-2 (MMP-2).279 On the other hand, in HFD-fed ...
Apical papilla stem cell-derived exosomes regulate lipid metabolism and alleviate inflammation in the MCD-induced mouse NASH model. Biochem Pharmacol. 2024;222: 116073. https://doi.org/10.1016/j.bcp.2024.116073. Article PubMed CAS Google Scholar Ohara M, Ohnishi S, Hosono H, Yamamoto K, ...
Transgenic APOE3*Leiden.huCETP (APOE3L.CETP) mice fed a high-fat diet (HFD) developed BS after 10 weeks. Then, inflammatory triggers were superimposed or not (control) for six more weeks. Mouse livers were analyzed with particular emphasis on hallmarks of inflammation which were defined in ...
To check diet effect and exercise plus diet effect on DNA methylation of GAB2 in NASH, DNA methylation profiling by EpiTyper MassARRAY and relative mRNA expression profiling by qRT-PCR were conducted for MCD, HFD, LFD and ELFD groups. Diet effect on DNA methylation and mRNA expression of GAB...
To achieve this goal, and considering that PTP1B-deficient mice are protected against obesity-induced NAFLD, an MCD dietary challenge was used as a preclinical model of non-obese NASH [26]. Although the MCD diet has been criticized because it causes body weight loss [27], it rapidly ...
Ectopic lipid accumulation and inflammation are the essential signs of NASH. However, the molecular mechanisms of ectopic lipid accumulation and inflammation during NASH progression are not fully understood. Here we reported that hepatic Wilms' tumor 1-a
The mouse models were mainly induced by a high-fat diet (HFD) (71.9%) and a methionine-choline deficient (MCD) diet (12.3%). Animal studies consisted of 85.9% NAFLD and 14.1% NASH models. In the 57 studies using animal models, miRNA agonists/antagonists were mainly administered ...