DART-seq[44~46] 普通哺乳动物细胞(HEK293T) 10× Genomics SMART-seq2 View Download Figure 2 The sequencing methods for single-cell nascent transcriptome. A: The process of constructing a single-cell nascent transcriptome library. Nascent RNA from single cells is labeled with nucleoside analogs, ...
FLEP-seq: simultaneous detection of RNA polymerase II position, splicing status, polyadenylation site and poly(A) tail length at genome-wide scale by single-molecule nascent RNA sequencing Yanping Long, Jinbu Jia, Weipeng Mo, Xianhao Jin & Jixian Zhai Nature Protocols volume 16, pages 4355–...
GRO-seq (Global nuclear Run-On sequencing)是一种专门测量新生RNA的方法。该方法通过对细胞进行迅速冷冻,使细胞核内正在转录的复合体处于冻结状态,然后提取细胞核,加入Run on缓冲液,使其重新恢复RNA的转录。利用抗BrdU抗体提取带BrU标记的新生成RNA ,用于下一步高通量测序分析。 新生RNA的变化直接反映了转录调控的...
DART-seq[44~46] 普通哺乳动物细胞(HEK293T) 10× Genomics SMART-seq2 View Download Figure 2 The sequencing methods for single-cell nascent transcriptome. A: The process of constructing a single-cell nascent transcriptome library. Nascent RNA from single cells is labeled with nucleoside analogs, ...