Nascent RNA-FISH of X-linked genes in human fibroblast clonesdoi:10.6084/M9.FIGSHARE.4276373.V1Cantone, IreneCantone I. Nascent RNA-FISH of X-linked genes in human fibroblast clones. 2016. figshare.https://dx.doi.org/10.6084/m9.figshare.4276373.v1....
<abstract abstract-type="key-points"><sec>ObjectiveDetection and quantification of RNA synthesis in cells is a widely used technique for monitoring cell viability, health, and metabolic rate.After exposure to environmental stimuli, both
(Right panel) Merge of immunostaining (red) and FISH (green).doi:10.1002/9783527619504.ch44J.A. GeigerK.M. NeugebauerJohn Wiley & Sons, LtdGeiger JA, Neugebauer KM. 2005. Fluorescent detection of nascent transcripts and RNA-binding proteins in cell nuclei. In Handbook of RNA biochemistry (...
50, No. 3 https://doi.org/10.1093/nar/gkac022 Published online 20 January 2022 Dicer dependent tRNA derived small RNAs promote nascent RNA silencing Arianna Di Fazio†, Margarita Schlackow†, Sheng Kai Pong†, Adele Alagia and Monika Gullerova * Sir William Dunn ...
We observed a striking change in the staining pattern of smFISH in the presence of SS-blocking ASO; the nucleus fills up with intronic RNA, but the 5′ and 3′ regions of the intron are distinct entities, as evidenced by the fact that on average only 24%–48% of the 3′ regions ...
Transcript elongation by RNA polymerase is a dynamic process, capable of responding to a number of intrinsic and extrinsic signals. A number of elongation ... Fish,N Rachel,Kane,... - BBA - Gene Structure & Expression 被引量: 321发表: 2002年 YidC and Oxa1 Form Dimeric Insertion Pores ...
To determine whether IRSp53 is involved in the establishment of apical–basal polarity and lumen formation, IRSp53 was depleted in MDCK cells using CRISPR/Cas9, shRNA24, and siRNAs, and in Caco-2 cells using CRISPR/Cas9. Single MDCK-control and IRSp53-depleted cells were seeded onto Matrigel...
MicroRNA-33 encoded by an intron of sterol regulatory element-binding protein 2 (Srebp2) regulates HDL in vivo Proc Natl Acad Sci U S A, 107 (2010), pp. 17321-17326 CrossrefView in ScopusGoogle Scholar 5 Y. Lange, M.H. Swaisgood, B.V. Ramos, T.L. Steck Plasma membranes contain...
genome of a hydrozoan jellyfish,Clytia hemisphaerica, was reported12. The authors studied gene use across life cycle stages and revealed that medusa-specific transcription factors were associated with diverse neurosensory structures. Compared toClytia, the polyp-only hydrozoanHydrahas lost many of the...
Detection of nascent RNA, single-copy DNA and protein localization by immuno FISH in mouse germ cells and preimplantation embryos. Nature protocols. 2011; 6 :270–284.Namekawa SH, Lee JT. Detection of nascent RNA, single-copy DNA, and protein localization by immunoFISH in murine germ cells ...