Click-iT AHA for Nascent Protein SynthesisAnalysisKitsProteomics
I.Nat New BiolKIMURA, M. AND KIMURA, I.: Increase of nascent protein synthesis in neuromuscular junction of rat diaphragm induced by denervation. Nature 241, 114-115 (1973)Kimura, M. and I. Kimuka , Increase of nascent protein synthesis in neuromuscular junction of rat diaphragm induced by...
Synthesis runs counter to directional folding of a nascent protein domainFolding of individual domains in large proteins during translation helps to avoid otherwise prevalent inter-domain misfolding. How folding intermediates observed in vitro for the majority of proteins relate to co-translational folding...
Protein synthesis by ribosomes produces functional proteins but also serves diverse regulatory functions, which depend on the coding amino acid sequences. Certain nascent peptides interact with the ribosome exit tunnel to arrest translation and modulate themselves or the expression of downstream genes. H...
Encoded by human EEF2 Gene (EF-G/EF-2 Family), highly conserved 857-aa 95-kDa cytoplasmic Elongation Factor 2 is essential for protein synthesis and required for GTP-dependent ribosomal A-site to P-site translocation ofnascentprotein chains: peptidyl-tRNA is moved from the ribosome acceptor si...
Transient ribosomal attenuation coordinates protein synthesis and co-translational folding. Nat. Struct. Mol. Biol. 16, 274–280 (2009). Article CAS PubMed Google Scholar Komar, A. A., Lesnik, T. & Reiss, C. Synonymous codon substitutions affect ribosome traffic and protein folding during in...
Comparison of the Quant-iT Protein Assay and Other Conventional Assays Highly Selective Antibodies for GFP and RFP Analysis of newly transcribed, or nascent, RNA provides information that cannot be obtained from analysis using tradit...
In the endoplasmic reticulum (ER) of cultured porcine thyrocytes, newly synthesized thyroglobulin (Tg, the precursor in thyroid hormone synthesis) initially forms protein aggregates, which are dissolved into monomers and then assembled to dimers, before intracellular transport and secretion. However, stud...
We further defined a nucleolar, cell-cycle-regulated alt-protein that negatively regulates assembly of the pre-60S ribosomal subunit (MINAS-60). Depletion of MINAS-60 increases the amount of cytoplasmic 60S ribosomal subunit, upregulating global protein synthesis and cell proliferation. Mechanistically,...
Protein synthesis by ribosomes produces functional proteins but also serves diverse regulatory functions, which depend on the coding amino acid sequences. Certain nascent peptides interact with the ribosome exit tunnel to arrest translation and modulate