We have developed an imaging-based fluorescence assay for fast, sensitive, non-toxic, and non-radioactive detection of nascent protein synthesis for use with traditional fluorescence microscopy and high-content imaging platforms.Dani M. HillScott T. Clarke...
During translation, the ribosome influences nascent protein chains at different levels. First, it renders nascent chains soluble relative to the corresponding ribosome-released proteins, thereby supporting cotranslational events devoid of undesirable aggregation7. Second, the inner geometry of the ribosomal e...
Protein synthesis by ribosomes produces functional proteins but also serves diverse regulatory functions, which depend on the coding amino acid sequences. Certain nascent peptides interact with the ribosome exit tunnel to arrest translation and modulate themselves or the expression of downstream genes. H...
We further defined a nucleolar, cell-cycle-regulated alt-protein that negatively regulates assembly of the pre-60S ribosomal subunit (MINAS-60). Depletion of MINAS-60 increases the amount of cytoplasmic 60S ribosomal subunit, upregulating global protein synthesis and cell proliferation. Mechanistically,...
cytosol35,36. Thus, importins constitute an enduring chaperoning system that holds onto its substrates from synthesis in the cytosol until its release into the native context in the nucleus. A notable exception was Efr3 (Fig.1f), which is annotated as a plasma membrane protein. Nevertheless, ...
This assay of nascent RNA measures instantaneous transcription activity, rather than steady-state RNAs, allowing us to probe a role for m6A on the process of transcription. Consistent with previous studies (Core and Adelman, 2019), we observed a significant TSS proximal enrichment of PRO-seq ...
However, whether the cAMP/PKA pathway is involved in protein misfolding stress is not known. Using transcriptomics, we examined the response to protein misfolding stress and found upregulation of numerous stress gene functions and downregulation of many genes related to protein synthesis and other ...
Priced at ~$1 per qPCR assay, the Click-iT® Nascent RNA Capture Kit provides an affordable way to capture nascent RNA, enabling novel transcriptome research. Discovery, Not Just Recovery Incorporate Click-iT® metabolic analog...
Priced at ~$1 per qPCR assay, the Click-iT® Nascent RNA Capture Kit provides an affordable way to capture nascent RNA, enabling novel transcriptome research. Discovery, Not Just Recovery Incorporate Click-iT® metabolic analog...
A statistically significant elevation in the number of PMNE cases identified was a result of the heightened surveillance we initiated. = 3433, The result demonstrated a statistically insignificant probability, less than 0.001. Previous ipsilateral open carpal tunnel release (CTR) was documented in ten...