Oxford Nanopore Technologies Ltd.基因组DNA连接法建库(SQK-L Nanopore实验指南 基因组DNA连接法建库（SQK-LSK114）版本：GDE_9161_V114_REVC_29JUN2022 仅供研究使用 Oxford Nanopore Technologies Ltd.Gosling Building, Oxford Science Park, Edmund Halley Rd, Oxford, OX4 4DQ, United Kingdom ...
The appropriate device-specific SQK-LSK114 protocol from Oxford Nanopore Technologies should be followed for use of these reagents. 产品类别: Next Generation Sequencing Library Preparation Products 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 E7180S -20 NEBNext® FFP...
ligation sequencing kit v14 sqk-lsk114 a versatile sequencing kit optimised for modal raw read accuracy of q20+ (99%+) and long read singleplex samples. this uses our latest kit 14 chemistry preparation time: 60 minutes read length: = fragment length input amount: 1000 ng dsdna, 100+ ng...
reset filters multiplexing pcr-free sample type ligation sequencing kit v14 sqk-lsk114 a versatile sequencing kit optimised for modal raw read accuracy of q20+ (99%+) and long read singleplex samples. this uses our latest kit 14 chemistry preparation time: 60 minutes read length: = fragment ...
SQK-NBD114.24 Native Barcoding Kit 24 V14 $699.00 Nanopore SQK-LSK114-XL Ligation Sequencing Kit XL V14 $4,000.00 Nanopore SQK-LSK114 Ligation Sequencing Kit V14 $599.00 Nanopore EXP-SFE001 Short Fragment Eliminator Expansion $99.00 Nanopore ...
按照Oxford Nanopore Technologies(ONT)公司提供的cDNA-PCR(最新的为SQK-PCS114)建库文档执行,包括样品质量检测、文库构建、文库质量检测和文库测序等流程,以cDNA-PCR方式进行展示 (图3)。起始投入10ng 富集的RNA (polyA富集或者核糖体去除)或,500ng 总RNA。
使用 Nanopore 的连接测序试剂盒 (SQK-LSK110) 处理文库,并使用 GridION 上的 MinION 流通池 (FLO-MIN106D) 进行测序。将样品测序 72 小时,洗涤 2 次,并在 23 小时和 46 小时重新加载。用于人类 RRMS 的 BED 文件可以通过 ONT 的“自适应采样目录”(https://community.nanoporetech.com/adaptive_sampling_...
Three strains of Salmonella carrying MDR plasmids were sequenced using the ONT SQK-LSK114 kit with flow cell R10.4.1, and de novo genome assembly was performed with average read accuracy (Q>10) of 98.9%. Results and Discussion For a 5-Mb-long bacterial genome, finished genome sequences ...
Fresh frozen samples were sequenced using R.10.4 flow cells in combination with the SQK-Q20 early access kit with the base calling option super accuracy (Guppy version 6.1.5). For the CD3-isolated cell library samples, R.10.4.1 flow cells in combination with SQK-LSK114 chemistry and Guppy...
The SQK-LSK114 Ligation Sequencing Kit (ONT, Oxford, UK) was used for ONT library preparation. Sequencing was conducted on a PromethION instrument, utilizing an R10.4.1 flow cell (ONT, Oxford, UK). The Illumina libraries were prepared using a NEBNext Ultra II DNA Library Prep Kit for Ill...