型号:PromethION 品牌:PACBIO 产地:美国 类型:进口 原制造商:PacBio 状态:在售 指导价格:100~200万元 上市时间:2017年 英文名称:PacBio PromethION sequencer 优点:诺禾的Nanopore 测序平台采用的是通量最大的型号⸺PromethION,单芯片产出平均能达50 Gb,最高可超过100 Gb,而且平均 读长可达到25 Kb以上,Read ...
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Methods: Total DNA extracts from nasal swabs collected from 48 feedlot cattle presenting with clinical signs of bovine respiratory disease (BRD) were sequenced using the Nanopore PromethION sequencer. After 24 h of sequencing, genotypes were imputed and genomic estimated breeding values (GEBVs) ...
现阶段ONT 一共有5个测序平台:Flongle,MinION,GridION X5,PromethION和SmidgION(可以直接连接在手机上完成测序,还只是一个概念的东西)。现阶段很多实验室自行搭建ONT的测序平台:其实就是买点芯片、测序试剂盒,然后公司送一共MinION,再配一个性能好点的笔记本或图形工作站安装MinKNOW software,USB一连就可以美美的测...
54.按照测序芯片的操作指南,将构建好的文库加载到flow cells r9.4测序芯片上,将芯片插入promethion sequencer(oxford nanopore technologies,oxford,uk)上机测序48-72h,使用minknow软件进行测序。 55.实施例3数据分析 56.(1)测序数据质控与组装 57.nanopore测序的下机数据的原始数据格式为包含所有原始测序信号的二代fast...
MinION Sequencer 2. Solid-state nanopores Solid-state nanoporesor synthetic nanopores are made from solid-state materials such as silicon nitride, aluminum oxide, or carbon nanotubes. Synthetic nanopores can be designed to the exact requirements needed for sequencing. They are created using various met...
Full-length HLA class I genotyping with the MinION nanopore sequencer. Methods Mol. Biol. 1802, 155–162 (2018). Article CAS PubMed Google Scholar Liu, C. et al. Accurate typing of human leukocyte antigen class I genes by Oxford Nanopore sequencing. J. Mol. Diagn. 20, 428–435 (2018...
Full-length HLA class I genotyping with the MinION nanopore sequencer. Methods Mol. Biol. 1802, 155–162 (2018). Article CAS PubMed Google Scholar Liu, C. et al. Accurate typing of human leukocyte antigen class I genes by Oxford Nanopore sequencing. J. Mol. Diagn. 20, 428–435 (2018...
Oxford Nanopore’s ultra-high throughput sequencing system, PromethION. Partnering the NVIDIA A100 Tensor Core GPU technology with the PromethION device aims to deliver the world’s most powerful sequencer that supports real-time analyses at scale, and can also analyse any length fragment of DNA...
Highlights include release of new "Kit 12" and R10.4 flow cells for >Q20 raw read and around Q30 Duplex sequencing accuracy, and the new PromethION P2 device, a palm-sized, high-throughput sequencer that delivers the most accessible low-cost, high-output