the same anti-B7-H3 mAb (Supplementary Fig.1). Overall, our data show that the 4Ig, as the dominant isoform, is a target for developing immunotherapeutic strategies. B12(VHH)-CAR-T cells have dramatically higher antitumor potency than other constructs in multiple solid tumor models in our ...
Liposomes as drug vehicles have advantages, such as payload protection, tunable carrying capacity and improved biodistribution. However, due to the dysfunction of targeting moieties and payload loss during preparation, immunoliposomes have yet to be favo
Liposomes as drug vehicles have advantages, such as payload protection, tunable carrying capacity and improved biodistribution. However, due to the dysfunction of targeting moieties and payload loss during preparation, immunoliposomes have yet to be favo
was injected subcutaneously for 6 times during 3 months. A VHH phagemid library was constructed from the mRNAs of the immunized peripheral lymphocytes, with a titer around 2.5 × 108. Library screening was performed by the routine protocol of phage display, panning and colony ELISA35. Briefly...
Linkers of various lengths and conformational properties were chosen from a linker database and they were randomly attached to both ends of an anti-GFP nanobody to facilitate insertion. Functional fusion constructs capable of forming filaments on the surface of flagellin-deficient host cells were ...
Based on these flow cytometry experiments and the SPR data, nanobody 4hD29 was selected as the lead compound and its binding was further characterized on EndoC-βH1 cells where 4hD29 binding could be determined (p ≤ 0.01; n = 5) (Fig.5D,I). Finally, 4hD29 bound significant...
AbNatiV is trained on aligned sequences of native antibody from curated immune repertoires from the Observed Antibody Space (OAS) database35and other sources (Methods). The model is trained for ten epochs separately on human VH, Vκ, Vλ and camelid VHH sequences (roughly 2 million unique...
respectively. Data and binding parameters were analyzed using the MicroCal PeakITC software (Malvern). The integrated heat versus molar ratio plots of the Ty1:RBD interactions were obtained by subtracting the Ty1 dilution heat uptake from the binding data. The NP-VHH1:RBD data were only baselin...
The production and purification of Nb6E (previously named VHH05) has been described previously27. Briefly, BL21(DE3) E. coli were transfected via heat shock with plasmids encoding nanobodies of interest and grown in a medium (Terrific Broth) containing kanamycin (50 μg/mL). Transformed ...
A VHH antibody, also known as nanobody (Nb), is the antigen-binding fragment from camelid or shark heavy-chain antibody, which is the smallest antibody fragment with antigen affinity.25,26Nb alone is about 12–15 KDa, and composed of four conserved framework regions (FRs) and three hyper...