69and therefore the VHH-NJB2 nanobody detected tumors in both human and murine cancer models. Of note, the radiolabeled nanobody detectedpancreatic intraepithelial neoplasias(PanINs) in KPC mice. PanINs are precursor lesions that progress topancreatic ductal adenocarcinoma; at present, there is no ...
The production and purification of Nb6E (previously named VHH05) has been described previously27. Briefly, BL21(DE3) E. coli were transfected via heat shock with plasmids encoding nanobodies of interest and grown in a medium (Terrific Broth) containing kanamycin (50 μg/mL). Transformed ...
VHH fragments were amplified via nested PCR using specific primers (Supplementary Table 1), then cloned into the pComb phage vector and transformed into E. coil SS320 competent cells. The positive clones were sequenced, and the diversity of the antibody library analyzed with the calculated ...
Liposomes as drug vehicles have advantages, such as payload protection, tunable carrying capacity and improved biodistribution. However, due to the dysfunction of targeting moieties and payload loss during preparation, immunoliposomes have yet to be favo
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In order to reduce data redundancy and improve computational efficiency, all the nanobody sequences were clustered and analyzed using the MMseqs2, which is an efficient sequence alignment and clustering algorithm [30], [31]. The clustering was performed using the full VHH sequence, to retain as...
(CH2 and CH3) and one antigen-binding domain that is termed nanobody, or VHH, or single-domain antibody. Nanobodies are functionally equivalent to conventional antibodies’ antigen-binding fragments in recognizing target antigens. Their molecular weights are around 12 to 15 kDa, about one-tenth ...
Saerens, D.; Pellis, M.; Loris, R.; Pardon, E.; Dumoulin, M.; Matagne, A.; Wyns, L.; Muyldermans, S.; Conrath, K. Identification of a Universal VHH Framework to Graft Non-Canonical Antigen-Binding Loops of Camel Single-Domain Antibodies.J. Mol. Biol.2005,352, 597–607. [Go...
serum from the same alpaca undergoes affinity purification of VHH protein fragments, which are analyzed by LC-MS/MS. The resulting MS data is compared with the sequence database generated from DNA sequencing reads to determine the corresponding VHH sequence [168]. This approach eliminates the need...
Liposomes as drug vehicles have advantages, such as payload protection, tunable carrying capacity and improved biodistribution. However, due to the dysfunction of targeting moieties and payload loss during preparation, immunoliposomes have yet to be favo