Nanobody purificationNanobodies have recently been introduced to the field of photodynamic therapy (PDT) as a very promising strategy to target photosensitizers selectively to cancer cells. Nanobodies are known for their characteristic small size (15kDa), high specificity, and high binding affinities....
An affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads. Cat No:Publications yta77 Non visible text Nano-Secondary Alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647 ...
andT cellmarker CD3 are all promising candidates for targeting and activatingcytotoxic T lymphocytes(CTLs) to induce anti-tumor responses. GRP78,CD38, and GPC3 expression on cancer cells have also been targeted for nanobody expression andpurification, which provide useful cancer cell markers and pot...
1. Gram-scale production and purification. Biotechnol. Prog. 21, 205–220 (2005). Article CAS PubMed Google Scholar Wu, Y. R., Sefah, K., Liu, H. P., Wang, R. W. & Tan, W. H. DNA aptamer-micelle as an efficient detection/delivery vehicle toward cancer cells. Proc. Natl ...
Construction, expression and purification of the chromobody, 1053-EGFP. To construct pET28a-1053-EGFP, the EGFP fragment, cut from pEGFP-N1 (Clontech) by Hind III and Not I, replaced PE38 in pET28a-1053. The expression conditions were similar to 1053-PE38. Around 6 mg of pure ...
1. Gram-scale production and purification. Biotechnol. Prog. 21, 205–220 (2005). Article CAS PubMed Google Scholar Wu, Y. R., Sefah, K., Liu, H. P., Wang, R. W. & Tan, W. H. DNA aptamer-micelle as an efficient detection/delivery vehicle toward cancer cells. Proc. Natl ...
Nano-Caps are premium affinity resins optimized for the effective and economic one-step protein purification. They consist of Nanobodies/ VHHs conjugated to agarose beads. Benefits: Fast one-step protein purification at 4°C High binding capacity ...
Nano-Caps® are optimized for effective and cost-efficient protein purification. Nano-Caps consist of Nanobodies conjugated to agarose. Explore the range Nano-CaptureLigands Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-Capt...
Nano-Caps® are optimized for effective and cost-efficient protein purification. Nano-Caps consist of Nanobodies conjugated to agarose. Explore the range Nano-CaptureLigands Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-Capt...
The process includes: gene synthesis and vector construction (optional), cell-free expression and purification, and delivery of purified antibodies. Get a Quote Fig. 6 Cell-free VHH Synthesis Process References 1. Jovčevska I, Muyldermans S. The Therapeutic Potential of Nanobodies. BioDrugs...