Nanobody purificationNanobodies have recently been introduced to the field of photodynamic therapy (PDT) as a very promising strategy to target photosensitizers selectively to cancer cells. Nanobodies are known for their characteristic small size (15kDa), high specificity, and high binding affinities....
An affinity reagent for IP and purification of Myc-tagged proteins. It consists of a Myc Nanobody/ VHH coupled to agarose beads. Cat No:Publications yta77 Non visible text Nano-Secondary Alpaca anti-mouse IgG1, recombinant VHH, Alexa Fluor® 647 ...
Construction, expression and purification of the chromobody, 1053-EGFP. To construct pET28a-1053-EGFP, the EGFP fragment, cut from pEGFP-N1 (Clontech) by Hind III and Not I, replaced PE38 in pET28a-1053. The expression conditions were similar to 1053-PE38. Around 6 mg of pure ...
Nano-Caps® are optimized for effective and cost-efficient protein purification. Nano-Caps consist of Nanobodies conjugated to agarose. Explore the range Nano-CaptureLigands Nano-CaptureLigands are optimized to specifically immobilize human, mouse, and rabbit antibodies to streptavidin/avidin. Nano-Capt...
PurificationPurified by Nickel column. FormLiquid Storage buffer0.01M PBS, pH 7.4. Stability and StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. ...
andT cellmarker CD3 are all promising candidates for targeting and activatingcytotoxic T lymphocytes(CTLs) to induce anti-tumor responses. GRP78,CD38, and GPC3 expression on cancer cells have also been targeted for nanobody expression andpurification, which provide useful cancer cell markers and pot...
1. Gram-scale production and purification. Biotechnol. Prog. 21, 205–220 (2005). Article CAS PubMed Google Scholar Wu, Y. R., Sefah, K., Liu, H. P., Wang, R. W. & Tan, W. H. DNA aptamer-micelle as an efficient detection/delivery vehicle toward cancer cells. Proc. Natl ...
Purification Purified by Nickel column. Form Liquid Storage buffer 0.01M PBS, pH 7.4. Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. Not...
The production and purification of Nb6E (previously named VHH05) has been described previously27. Briefly, BL21(DE3) E. coli were transfected via heat shock with plasmids encoding nanobodies of interest and grown in a medium (Terrific Broth) containing kanamycin (50 μg/mL). Transformed ...
PCSK9 nanobody was obtained by Ni column affinity purification. The Ni column was washed first with ultrapure water and then with the lysis solution. The supernatant of the above-mentioned crushed PCSK9 nanobody expression bacteria was loaded to the Ni column at a flow rate of 1 mL/min; th...