A high diversity T7 phage display nanobody library was constructed for bio-panning of intact chicken bone marrow DCs to find DC-specific binding nanobodies. After three rounds of screening, 46 unique sequence phage clones were identified from 125 randomly selected phage clones. Several DC-binding...
Nanobody development process includes alpaca immunization, phage library construction, nanobody screening, expression, purification, and validation stages. After alpaca immunization, B lymphocytes are isolated from the peripheral blood, and total RNA is extracted and reverse-transcribed to cDNA. This cDNA...
The company provides services such as nanobody (single domain antibody) screening platform, mouse monoclonal antibody platform, CAR-T development and verification, yeast display library platform, etc. The team is engaged in recombinant viruses (adenoviru
an anti-proteome nanobody library approach yields a specific immunoassay for trypanosoma congolense diagnosis targeting .一个anti-proteome nanobody库方法产生一 文档格式: .pdf 文档大小: 722.17K 文档页数: 24页 顶/踩数: 0/0 收藏人数: 0
上海玉博生物科技有限公司做为kerafast代理商之产品EF0014-FP- Yeast-Display Nanobody Library (NbLib) Datasheet 1 vial (>2.5x10^9 cells)的产品销售公司,专业经营原装进口抗体(单克隆、多克隆)、重组蛋白、检测试剂盒、标准品、细胞因子、胎牛血清、细胞培养类等生物试剂以及耗材。
Here we describe a nanobody library comprising 12 unique nanobodies to the Y complex and Nic96 fromS. cerevisiae. Nanobodies are single-domain (VHH) antibody fragments derived from camelid heavy-chain only antibodies. Nanobodies are excellent tools both in vitro and in vivo, as they are small...
The company provides services such as nanobody (single domain antibody) screening platform, mouse monoclonal antibody platform, CAR-T development and verification, yeast display library platform, etc. The team is engaged in recombinant viruses (adenoviru
Nanobody library generation Nanobody libraries were constructed by overlapping polymerase chain reaction (PCR), combining a series of degenerated primers to generate the DNA encoding full-length nanobody. Degenerated bases were used to randomize CDR1, 2, and 3. Three different lengths were constructe...
The company provides services such as nanobody (single domain antibody) screening platform, mouse monoclonal antibody platform, CAR-T development and verification, yeast display library platform, etc. The team is engaged in recombinant viruses (adenoviru
(see Supplementary TableS1), as linker candidates, primarily based on thelinkerdbwwwlinker database27. The aGFP_ENH nanobody, with linkers from the chosen set randomly attached to its both ends (Fig.1b-c), was inserted into flagellin replacing the D3 domain. In this way a library of ...