该试剂盒用于从人PBMC中快速、高效分离未经标记的初始CD4 T细胞。通过用生物素化的CD45RO、CD8、CD14、CD15、CD16、CD19、CD25、CD34、CD36、CD56、CD123、抗TCRγ/δ、抗HLA-DR和CD235a抗体孵育PBMC,然后用抗生物素磁珠进行磁标记,从而去除记忆性CD4 T细胞和非CD4T细胞。 优点:...
通过去除记忆CD4+T细胞和非CD4+T细胞来分离小鼠初始CD4+T细胞。 小鼠脾脏或淋巴结的单细胞悬液用生物素化的单克隆抗体混合物(主要试剂)标记,而抗生物素的磁珠作为辅助标记试剂间接磁性标记细胞。 同时,将CD44 磁珠添加到标记记忆T细胞中。 磁性标记过程中无需清洗步骤。 通过将磁性标记的非靶细胞保留在MACS分离器磁场...
naive T cell,也称作未致敏T细胞,在胸腺中发育成熟迁移至外周淋巴组织中(如脾脏、淋巴结)。其未接触抗原刺激前处于相对静止状态,在胸腺中发育的一类带有CD4、TCR/CD3、CD28、CD62L、CCR7等表面分化抗原的T细胞。其通过抗原刺激或树突状细胞(Dendritic cell,DC)的递呈作用,可进一步分化成Th1细胞、Th2细胞、Th17细胞、...
MojoSort™ Human CD4 Naïve T Cell Isolation Kit - Non-CD4+ Naïve T cells are depleted by incubating the sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads.
MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit - Non CD4+ Naïve T cells are depleted by incubating your sample with the biotin antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads.
Moreover, naive population size predicted the size and TCR diversity of the primary CD4+ T cell response after immunization with relevant peptide. Thus, variation in naive T cell frequencies can explain why some peptides are stronger immunogens than others....
英文别名: BD IMag Human Naive CD4 T Cell Enrichment Set - DM中文名: 中文别名: CBNumber: CB315304406 分子式: 分子量: 0 MOL File: Mol file 化学性质 安全信息 用途 供应商 1 BD IMag Human Naive CD4 T Cell Enrichment Set - DM化学性质 ...
Food allergy poses a significant clinical and public health burden affecting 2–10% of infants. Using integrated DNA methylation and transcriptomic profiling, we found that polyclonal activation of naive CD4+ T cells through the T cell receptor results i
Yet, the use of separated T cell populations revealed that both CD4+ and CD8+ T cells express this function in adults whereas the CD4 subset is primarily involved in lysis by a Fas-based mechanism in cord. Altogether these results suggest that distinct T cell subsets might be recruited to ...