Here, we provided several lines of evidence that link the NAC domain-containing protein GmNAC6 to the NRP-mediated cell death response. Like NRPs, GmNAC6 is synergistically activated by a combination of ER stress and osmotic stress signals and induces a senescence-like response in planta and ...
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A structural view of the conserved domain of rice stress-responsive NAC1. Protein Cell. 2011;2(1):55–63. Article PubMed PubMed Central Google Scholar Ernst HA, Olsen AN, Larsen S, Lo Leggio L. Structure of the conserved domain of ANAC, a member of the NAC family of transcription ...
Interestingly, six of the top eight most connected TFs are NAC domain-containing proteins (Glyma.19G108800, Glyma.01G051300, Glyma.02G109800, Glyma.12G221400, Glyma.16G043200 and Glyma.13G280000), which all belong to the SNAC-B/NAP sub-family29. In total, 11 members of this subfamily...
The NAC domain protein family is a larger plant-specific TF family. The family name is based on the three proteins: no apical meristem (NAM), ATAF1–2, and cup-shaped cotyledon (CUC), which all possess a similar DNA-binding domain [5, 6]. Typically, the structure of NAC proteins can...
To test the transcriptional activity of ClNAC68, we generated N- and C-terminal ClNAC68 constructs containing the NAC domain (aa 1–197) and transcription domain (aa 198–319), respectively (Fig. 1D). Then, Y2H and dual-luciferase reporter assays were performed. For the Y2H assay, all ...
Protein concentration was determined in the TBS and SDS soluble fractions using a BCA assay (Pierce, Rockford, IL). Samples were mixed with 2X Laemmli sample buffer containing 10% β-mercaptoethanol and boiled for 5 minutes. 5 μg of protein of TBS fractions, 10 μg of protein of SDS ...
The NAC domain-containing protein sequences were obtained from the site http://www.phytozome.net/, using the NAC domain of GmNAC3 (AAY46123) as the prototype sequence for searching. All the six previously described GmNACs could have been used as guidance for searching as they all harbor a ...
During pathogen attack, the receptor-like protein kinases (RLKs) are the first key regulator proteins of pathogen-associated molecular patterns-triggered immunity (PTI). Among the identified kinases, many of them belong to leucine-rich repeat domain-containing RLKs, which regulate a wide variety of...
Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Sci. 2009;324:1068–71. Pizzio GA, Rodriguez L, Antoni R, Gonzalez-Guzman M, Yunta C, Merilo E, et al. The PYL4 A194T mutant uncovers a key role of PYR1-LIKE4/PROTEIN PHOSPHATASE 2CA...