Interestingly, six of the top eight most connected TFs are NAC domain-containing proteins (Glyma.19G108800, Glyma.01G051300, Glyma.02G109800, Glyma.12G221400, Glyma.16G043200 and Glyma.13G280000), which all belong to the SNAC-B/NAP sub-family29. In total, 11 members of this subfamily...
A structural view of the conserved domain of rice stress-responsive NAC1. Protein Cell. 2011;2(1):55–63. Article PubMed PubMed Central Google Scholar Ernst HA, Olsen AN, Larsen S, Lo Leggio L. Structure of the conserved domain of ANAC, a member of the NAC family of transcription ...
The NAC domain protein family is a larger plant-specific TF family. The family name is based on the three proteins: no apical meristem (NAM), ATAF1–2, and cup-shaped cotyledon (CUC), which all possess a similar DNA-binding domain [5, 6]. Typically, the structure of NAC proteins can...
During pathogen attack, the receptor-like protein kinases (RLKs) are the first key regulator proteins of pathogen-associated molecular patterns-triggered immunity (PTI). Among the identified kinases, many of them belong to leucine-rich repeat domain-containing RLKs, which regulate a wide variety of...
Protein concentration was determined in the TBS and SDS soluble fractions using a BCA assay (Pierce, Rockford, IL). Samples were mixed with 2X Laemmli sample buffer containing 10% β-mercaptoethanol and boiled for 5 minutes. 5 μg of protein of TBS fractions, 10 μg of protein of SDS ...
We have found that BCL6 is a co-factor in NAC1-containing transcription complexes. NAC1 interacts with BCL6 via its C-terminal BEN domain and forms a transcription complex that binds to the promoter region of the NAC1 target gene, FOXQ1. Moreover, we showed that NAC1 and BCL6 are coo...
[ 71] Wang XꎬGoregaoker S PꎬCulver J N.Interaction of the To~ baccomosaic virus replicase protein with a NAC domain transcriptionfactor is associated with the suppression of 第 6 卷第 3 期 文锦芬ꎬ等:转录因子 DREB,ERF 和 NAC 在介导植物响应生物和非生物胁迫中的作用 59 systemic ...
Protein sequence comparison showed that ZmNAC41 and ZmNAC100 are closely related; sharing 78% similarity of the whole sequence and 87% in the NAC domain. Thus, these two transcription factors belong to clade G, as revealed by a phylogenetic analysis (Figure 5). Checking the gene duplication ...
(version 3.2.1) was used to search for NAC proteins from all protein sequences of sunflower. The obtained proteins were verified in the CCD database (https://www.ncbi.nlm.nih.gov/cdd/). The remaining protein sequences with the complete NAC domain were aligned using the ClustalW method ...
The C-terminal domains (amino acids 148-317) of ANAC019 and ANAC055, respectively, were fused to the GAL4 DNA-binding domain to examine their abil- ity to activate transcription from the GAL4 upstream activation sequence (UAS) and thereby promote yeast growth. The yeast cells containing pBD...