N6-Methyladenosine (m6A) RNA modification is present in messenger RNAs (mRNA), ribosomal RNAs (rRNA), and spliceosomal RNAs (snRNA) in humans. Although mRNA m6A modifications have been extensively studied and s
Protection from harmful pathogens depends on activation of the immune system, which relies on tight regulation of gene expression. Recently, the RNA modification N6-methyladenosine (m6A) has been found to play an essential role in such regulation. Here,
4,18 Accordingly, ectopic expression of METTL3-wt, but not METTL3-mut1, promoted colony formation in CRC cells (Figure 3G), corroborating that m6A methyltransferase activity of METTL3 is essential for CRC growth. METTL3 Mediates N6-Methyladenosine Messenger RNA Methylation in Colorectal Cancer To...
其中,N6-甲基腺苷(N6-methyladenosine, m6A)修饰的生物学作用逐渐受到重视[1]。据估计[2],细胞中25%的mRNA存在m6A修饰。而长链非编码RNA(lncRNA)、微小RNA(miRNA)和病毒转录产物等RNA分子中也存在m6A修饰现象。自1970年起,RNA分子中的m6A甲基化现象被陆续发现,当时的m6A检测主要依靠水解或核酸酶消化放射性标记的RN...
Macrophage polarization is associated with the RNA epigenetic alteration N6-methyladenosine (m6A) RNA methylation. However, the specific function and underlying mechanisms of m6A methylation in the role of macrophage polarization in RA remain to be elucidated. The mRNA expression levels of m6A methylase...
N6-甲基腺嘌呤(N6-methyladenosine,m6A)是发生在RNA腺嘌呤(A)碱基第6位氮原子上的甲基化,主要由甲基转移酶(Writers)、去甲基化酶(Erasers)和相关结合蛋白(Readers)共同调控,参与RNA转录、剪接、加工、翻译和衰变等多种生物学过程[1-3]。肝脏是调控机体新陈代谢的主要器官,m6A甲基化参与肝脏疾病的发生、发展过程(...
N6-methyladenosine (m6A) mRNA modification is essential for mammalian and plant viability. The U6 m6A methyltransferases in other species regulate S-adenosylmethionine (SAM) homeostasis through installing m6A in pre-mRNAs of SAM synthetases. However, U6 m6A methyltransferase has not been characterized in...
YTH N6甲基腺苷RNA结合蛋白(YTH N6-methyladenosine RNA binding protein,YTHDF)1可与mRNA终止密码子附近的m6A位点结合,从而促进RNA翻译。而YTHDF2通过募集一种叫作CCR4-NOT的腺苷酸酶复合物,来促进m6A修饰的转录产物的降解。YTHDF3能够与YTHDF1结合,共同作用...
N6-甲基腺苷(N6-methyladenosine,m6A)甲基化:是指发生在RNA腺嘌呤N6位点上的甲基化修饰,是一种动态可逆的修饰模式,m6A修饰在转录后水平上调控RNA翻译、可变剪切、转运、定位和降解等,是真核生物mRNA中最丰富的表观遗传修饰,在调控各种细胞的分化、修复、侵袭和凋亡中起重要作用。
RNA modification in the form of-methyladenosine (mA) regulates nearly all the post-transcriptional processes. The asymmetric mA deposition suggests that regional methylation may have distinct functional consequences. However, current RNA biology tools do not distinguish the contribution of individual mA ...