Palmitoylation is the post-translational covalent attachment of a palmitoyl group to amino acid residues. Cysteine palmitoylation, or S-palmitoylation, is the major form of palmitoylation. Due to the labile nature of the thioester linkage, S-palmitoylation is reversible and dynamic, making it an ...
We provide direct biochemical evidence that Hhat is a PAT with specificity for attaching palmitate via amide linkage to the N-terminal cysteine of Shh. Other palmitoylated proteins (e.g. PSD95 and Wnt) are not substrates for Hhat, and Porcupine, a putative Wnt PAT, does not palmitoylate ...
10.7554/eLife.11306.001Dynamic changes in protein S-palmitoylation are critical for regulating protein localization and signaling. Only two enzymes - the acyl-protein thioesterases APT1 and APT2 ??? are known to catalyze palmitate removal from cytosolic cysteine residues. It is unclear if these enzy...
L-Cysteine, S-(2,3-dihydroxypropyl)-N-(1-oxohexadecyl)-, methyl ester GLYCINE, S-[2,3-BIS[(1-OXOHEXADECYL)OXY]PROPYL]-L-CYSTEINYL-L-ALANYL- L-Lysine,S-[2,3-bis[(1-oxohexadecyl)oxy]propyl]-N-(1-oxohexadecyl)-L-cysteinyl-L-seryl-L-lysyl-L-lysyl-L-lysyl- ...
The N-Palmitoylation is carried out by attachment of palmitic acid to an N-terminal cysteine. Due to the association of N-Palmitoylation with various biological functions and diseases such as Alzheimer's and other neurodegenerative diseases, its identification is very important. Objective: The in ...
Brevetoxins (BTXs) are a class of cyclic polyether toxins produced by the dinoflagellate Karenia brevis. These substances are subject to extensive conjugative metabolism in shellfish. BTX-B forms a conjugate with cysteine and is oxidized and reduced to yield BTXB2, which is further modified by ...
Upstream of mCherry, the Drosophila Hh export signal sequence (codons 1 to 84) was followed by the Drosophila Hh extended N-terminal peptide with a functional palmitoylation acceptor cysteine (p, codons 85 to 98). C-terminal to the mCherry fluorophore, the three most C-terminal amino acids ...