more flexible N-glycosylation biocatalysts for use in glycoengineering applications and also in-depth understanding of the role of Gln469 in catalysis and substrate binding, a set of mutants with deletions of Gln469 and/or its neighboring residues were herein constructed by overlap extension PCR. The...
more flexible N-glycosylation biocatalysts for use in glycoengineering applications and also in-depth understanding of the role of Gln469 in catalysis and substrate binding, a set of mutants with deletions of Gln469 and/or its neighboring residues were herein constructed by overlap extension PCR. The...
1C). RT-PCR analysis confirmed successful expression of the newly introduced B4GalT1 and ST6Gal1 transcripts (Supplementary Fig. S3B). Compared to the MGAT5 targeted integration pools, additional expression of B4GalT1 and/or ST6Gal1 in the combinatorial pools resulted in a 10% reduction in ...
In addition, using reverse transcriptase-PCR, T. gondii ppGalNAc-T3 mRNA was equivalently expressed during the tachyzoite and bradyzoite developmental stages. The identification of T. gondii ppGalNAc-T3 as a functional "follow-up" glycopeptide glycosyltransferase further confirms that this human ...