www.nature.com/scientificreports OPEN Improvement of the glycoproteomic toolbox with the discovery of a unique C-terminal Received: 10 May 2017 Accepted: 29 August 2017 Published: xx xx xxxx cleavage specificity of flavastacin for N-glycosylated asparagine Alexander Pralow1, Marcus Hoffmann1, ...
Sequential immunoprecipilalion studies with the MoAb 44G4, which recognizes the o- and N-glycosylated homodimer endoglin, showed that both MoAbs recognize ... PJ O'Connell,A Mckenzie,N Fisicaro,... - 《Clinical & Experimental Immunology》 被引量: 224发表: 1992年 Production of a New Murine...
Hyper-N-glycosylated SEL1L3 as auto-antigenic B-cell receptor target of primary vitreoretinal lymphomasMichelle Elbert, Frank Neumann, Maximilian Kiefer, Konstantinos Christofyllakis, Benedikt Balensiefer, Igor Kos, Gabi Carbon, Dominic Kaddu-Mulindwa, Joerg Thomas Bittenbring, Natalie ...
The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of...
new methods based on glycoproteins/ glycopeptides enrichment and mass spectrum techniques,scientists have been able to identify glycosylated sites in different tissues.Besides,the discovery of novel sequence patterns for N-glycosylation will help to understand the process of N-glycosylation better in ...
[Methods in Molecular Biology] Mass Spectrometry of Glycoproteins Volume 951 || Stable Isotope Labeling of N-Glycosylated Peptides by Enzymatic Deglycosyla... JJ Kohler,SM Patrie 被引量: 0发表: 2013年 chapter 14 stable isotope labeling of n -glycosylated peptides by enzymatic deglycosylation for...
elegans. Glycosidase treatment and site-directed mutagenesis confirmed that CeCNX-1 is N-glycosylated at two asparagine residues of Asn203 and Asn571. When transgenic animals from cnx-1 mutant were generated, a glycosylation defective construct failed to rescue phenotypes of cnx-1 mutant suggesting ...
However, only three N-linked glycosylated proteins, galectin-3 binding protein, lysosome associated membrane glycoprotein 1, and oxygen regulated protein, were identified in all three breast cancer cell lines. In addition, MCF-7 cells shared an additional 3 proteins with MDA-MB-453. Interestingly,...
Thus, the effect of N-glycosylation on recombinant protein production has not been fully elucidated. In this study, we investigated the effects of the fusion of a highly N-glycosylated peptide on recombinant protein expression in plants. We fused leptin which is important hormone for regulating ...
The present invention relates to recombinant N-glycosylated proteins, comprising one or more introduced N-glycosylated optimized amino acid sequence(s), nucleic acids encoding these proteins as well as corresponding vectors and host cells. In addition, the present invention is directed to the use of...