将以上机制转移到大肠杆菌中,实现 N-糖基化重组蛋白,可重塑合成寡糖链及重组蛋白修饰,将在糖疫苗生产、糖基化修饰药物蛋白等方面具有巨大应用潜力。 Schematic diagram of the main mechanism of N-glycosylated recombinant protein production via Escherichia coli A: OST-dependent N-glycosylation system; B: OST-...
The protein-DNA crosslinking capability of cis-dichloro diammineplatinum has been exploited to check the intranuclear location of N-glycosylated proteins. When intact liver cells were treated with this reagent, a number of glycoproteins, recognized by Concanavalin A, have been shown to become ...
Protein–protein interaction (PPI) analysis of N-glycoprotein Based on the STRING or InAct databases, Cytoscape software was used to construct a protein interaction network diagram for the N-glycoprotein corresponding to the N-glycosylated peptides with differential abundances identified in each comparison...
Schematic diagram of the production of N-glycosylated recombinant proteins using Escherichia coli A:Recombinant protein pathway modified by N-glycosylation in Escherichia coli; B: Cell-free glycoprotein synthesis pathway O-糖基化修饰可以增加治疗性蛋白的稳定性和体内循环时间。虽然糖基化修饰对每种蛋白质的...
Schematic diagram of the production of N-glycosylated recombinant proteins using Escherichia coli A: Recombinant protein pathway modified by N-glycosylation in Escherichia coli; B: Cell-free glycoprotein synthesis pathway O-糖基化修饰可以增加治疗性蛋白的稳定性和体内循环时间。虽然糖基化修饰对每种蛋白质...
The other improvement in methodology was the sequential LC/MS/MS analysis for determining more glycoprotein structures, which consisted of quantitative proteomic, glycoproteomic, and deglycosylated proteomic analyses. In addition to abundant glycoproteins, such as integrins and laminins, the method also ...
Salt tolerance of Arabidopsis thaliana requires maturation of N-glycosylated proteins in the Golgi apparatus Protein N-glycosylation in the endoplasmic reticulum (ER) and in the Golgi apparatus is an essential process in eukaryotic cells. Although the N-glycosylat... JS Kang,J Frank,HK Chang,.....
1). More than half of the identified sites (52%) were previously annotated automatically at every N-glycosylation consensus sequence due to the fact that the protein was known to be N-glycosylated. Furthermore, 10 sites were found on proteins not known to be glycosylated at all or for ...
In a first aspect the present invention provides a recombinant N-glycosylated protein, comprising one or more of the following N-glycosylated optimized amino acid sequence(s): D/E-X-N-Z-S/T, (optimized consensus sequence) wherein X and Z may be any natural amino acid except Pro, and ...
Hen ovalbumin (OVA) is known to exist as a singly N-glycosylated form with a glycan chain on Asn-292 in egg white. Previous studies showed that di-N-glycos... T Suzuki,K Kitajima,Y Emori,... - 《Proceedings of the National Academy of Sciences of the United States of America》 被引...