Nanba H,Ikenada Y,Yamada Y, et al.Immobilization of N-carbamyl-D-amino acid amidohydrolase.Biosci. Biotechnol. Biochem. 1998Nanba, H., Ikenaka, Y., Yamada, Y., Yajima, K., Takano, M., Ohkubo, K., Hiraishi, Y., Yamada, K., and Takahashi, S., Immobilization of N -carbamyl- D...
Background: N-carbamyl- d-amino acid amidohydrolase (DCase) catalyzes the hydrolysis of N-carbamyl- d-amino acids to the corresponding d-amino acids, which are useful intermediates in the preparation of β-lactam antibiotics. To understand the catalytic mechanism of N-carbamyl- d-amino acid hydr...
To facilitate the easier production of d-amino acids using N-carbamyl-d-amino acid amidohydrolase (DCase) in an immobilized form, we improved the enzymatic thermostability of highly soluble DCase-M3 of Ralstonia pickettii using directed mutagenesis. Six novel mutation sites were identified in this st...
Nanba H, Ikenaka Y, Yamada Y, Yajima K, Takano M, Takahashi S (1994) Production of thermotolerant N-carbamyl-D-amino acid amidohydrolase by recombinant Escherichia coli. J Biosci Bioeng 87:149-154Nanba H, Ikenaka Y, Yamada Y, Yajima K, Takano M, Takahashi S (1999) Production of ...
N-Carbamyl-D-amino acid amidohydrolasethermotolerantN-Carbamyl-D-amino acid amidohydrolase (DCase), in which amino acid residues were substituted by mutation, followed by the selection based on thermotolerance, showed improved thermostability, by 5 deg or 10 deg C, compared to the native DCase. ...
Directed Evolution of N-Carbamyl-D-amino AcidAmidohydrolase for Simultaneous Improvement of Oxidativeand Thermal Stability.. Ki-Hoon Oh, 等.Biotechnol. Prog,Vol.18 No.3. 2002Oh KH, Nam SH, Kim HS (2002a) Directed evolution of N- carbamoyl-D-amino acid amidohydrolase for simultaneous ...
Nanba H,Ikenaka Y,Yamada Y,et al.Isolation of Agrobacterium sp Strain KNK712that Produces N-Carbamyl-D-amino Acid Amidohydrolase,Cloning of the Gene for this Enzyme,and Properties of the Enzyme. Biosci.Biotechnol.Biochem . 1998Nanba, H., Ikenaka, Y., Yamada, Y., Yajima, K., Takano, ...
Oh KH,Nam SH,Kim HS.Directed evolution of N-carbamyl-D-amino acid amidohydrolase for simultamneous improvement of oxidative and thermal stability. Biotechnology Progress . 2002Oh KH,Nam SH,Kim HS.Directed evolution of N-carbamyl-D-amino acid amidohydrolase for simultamneous improvement of ...
doi:10.1002/1522-2640(200205)74:5<675::AID-CITE675>3.0.CO;2-KO. VielhauerM. WernerM. PietzschM. SiemannC. SyldatkJohn Wiley & Sons, Ltd.Chemie Ingenieur TechnikHerrmann, R, Schwieger, W, Scharf, O, Toufar, H, Ziberi, B, Gill, W (2002) Chem. Ing. Tech. 74: pp. 650...
Im- proving the thermostability of N-carbamyl-D- amino acid amidohydrolase by error-prone PCR. Appl Microbiol Biotechnol 2009; 82: 279-285.Hong Yu,Jian Li,Dalong Zhang,Yunliu Yang,Weihong Jiang,Sheng Yang. Improving the thermostability of N-carbamyl-d-amino acid amidohydrolase by error-...