Nanba H,Ikenada Y,Yamada Y, et al.Immobilization of N-carbamyl-D-amino acid amidohydrolase.Biosci. Biotechnol. Biochem. 1998Nanba, H., Ikenaka, Y., Yamada, Y., Yajima, K., Takano, M., Ohkubo, K., Hiraishi, Y., Yamada, K., and Takahashi, S., Immobilization of N -carbamyl- D...
Crystal structure of N-carbamyl-D-amino acid amidohydrolase with a novel catalytic framework common to amidohydrolases. Structure 8, 729–737 (2000). Article CAS Google Scholar Buddelmeijer, N. & Young, R. The essential E. coli Apoliprotein N-acyltransferase (Lnt) exists as an extra...
Background: N-carbamyl- d-amino acid amidohydrolase (DCase) catalyzes the hydrolysis of N-carbamyl- d-amino acids to the corresponding d-amino acids, which are useful intermediates in the preparation of β-lactam antibiotics. To understand the catalytic mechanism of N-carbamyl- d-amino acid hydr...
摘要: N-Carbamyl-D-amino acid amidohydrolase (N-carbamoylase), which is currently employed in the industrial production of unnatural D-amino acid in conjunction with D-hydantoinase, has low oxidative and thermal stability, and low expression. First, we...
The mutated enzymes are expected to be applied to the development of a fully enzymatic process for the industrial production of d-amino acids. 展开 关键词: N-carbamyl-d-amino acid amidohydrolase Thermostability d-amino acid Error-prone PCR ...
N-甲基-D天冬氨酸受体2B亚基镇痛疫苗的构建及其镇痛效应的研究 12. Immobilization of N-carbamyl-D-amino acid amidohydrolase N-氨甲酰基-D-氨基酸酰胺水解酶的固定化工艺 13. Synthesis of N-acetyl-aspartyl-glutamate-a kind of nerve dipeptide 神经二肽N-乙酰天门冬氨酰谷氨酸的合成 14. Effect of AcS...
Immobilization of N-carbamyl-D-amino acid amidohydrolase N-氨甲酰基-D-氨基酸酰胺水解酶的固定化工艺 9. The Further Research of Mouse N5-glutamine Methyltransferase Gene mHemk 小鼠N5-谷氨酰胺甲基转移酶基因mHemk的进一步研究 10. Progresses on Synthesis of N-methylaniline and N,N-dimethylaniline ...
Property of immobilized N-carbamyl-D-amino acid amidohydrolase 以TJS环氧基树脂作为载体,对N-氨甲酰基-D-氨基酸酰胺水解酶进行固定化研究。 更多例句>> 4) D-Carbamoylase N-氨甲酰-D-氨基酸酰胺水解酶 1. In the reaction process, the substrate DL-5'- monosubstitued hydantoin is enantioselectively...
Improving the thermostability of N -carbamyl-d -amino acid amidohydrolase by error-prone PCR To facilitate the easier production of d-amino acids using N-carbamyl-d-amino acid amidohydrolase (DCase) in an immobilized form, we improved the enzymatic... H Yu,J Li,D Zhang,... - 《Applied Mi...
Agrobacterium radiobacter N-carbamyl D-amino acid amidohydrolase 1.95 的晶體結構 Agrobacterium radiobacter CCRC 14924菌種中的N-carbamyl D-amino acid amidohydrolase(Nca)可應用於D型胺基酸的生產,此酵素目前已可以在 Escherichia coli JM109中大量表現.Nca的晶體屬於單斜晶系,其空間群為P21,單位晶胞的參數為a =...