The present invention provides a modified microorganism which this enzyme is overexpressed polypeptides, and isolated with a L- amino -N- acyltransferase activity. Substrates of this enzyme include analogs or derivatives thereof and methionine primarily. Overexpression in sulfur-containing amino acid-...
as judged by a 32Pi incorporation assay. In order to examine N-acyltransferase activity directly, the substrate [alpha-32P]UDP-3-O-(R-3-hydroxymyristoyl)-GlcN was synthesized enzymatically. N-Acyltransferase specific activity in RL-25 extracts was reduced to less than *0% of wild-type. ...
N-acyl transferase activity of LntPae and LntEco was monitored as a shift in the lipopeptide (fibroblast stimulating ligand; FSL-1-biotin; molecular weight (MW), 2,247 Da) substrate band position on an SDS-PAGE towards higher MW values. Reaction mixtures contained 20-100 nM enzyme, 500...
BCG_2070c is identical to an ORF with proven N-acyltransferase activity since M. tuberculosis Lnt complemented the M. smegmatis lnt deletion mutant and all three residues of the catalytic triad essential for Lnt function in E. coli are conserved. Lnt activity of BCG_2279c may be buried by ...
The isopenicillin-N acyltransferase of Penicillium chrysogenum catalyzes the conversion of the biosynthetic intermediate isopenicillin N to the hydrophobic penicillins. The isopenicillin-N acyltransferase copurified with the acyl-CoA:6-aminopenicillanic acid (6-APA) acyltransferase activity which transfers ...
4B). A previous mutational study20 has shown P346 to be essential for Lnt activity at higher growth temperatures. Another consequence of the mobile nature of this arm is the slight movement of the essential residue E343 that is at one of the joints allowing movement between the 2 ...
Using localized mutagenesis of whole cells, we have isolated a temperature-sensitive UDP-N-acetylglucosamine acyltransferase mutant of Escherichia coli that loses all detectable acyltransferase activity and quickly dies after a shift fro... SM Galloway,CR Raetz - 《Journal of Biological Chemistry》 被引...
Assay of PAM Activity Reactions at 37.0 ± 0.1° are initiated by the addition of PAM (10–50 μg) into 2.4 ml of 100 mM MES/NaOH, pH 6.0, 30 mM NaCl, 1% (v/v) ethanol, 0.001% (v/v) Triton X‐100, 1.0 μM Cu(NO3)2, 5.0 mM sodium ascorbate, and the bile acid glycine ...
[6],andalsoexhibitedanalgesic,anxiolytic,andanti-allergicactivityinvariousmodelsystems[4,7–8].Thebiologicalrolesfortheotherknownlong-chainN-acylserotoninshaveyettobedetermined.Recently,Verhoeckxetal.[9]identifiedandquantifiedtheendogenousamountsoflong-chainN-acylserotoninsinthedifferentregionsoftheporcine...
Here we describe a sensitive fluorescence-based assay to monitor bacterial apolipoprotein N-acyltransferase activity.Similar content being viewed by others Fluorescence anisotropy assays for high throughput screening of compounds binding to lipid II, PBP1b, FtsW and MurJ Article Open access 14 April ...