The present invention provides a modified microorganism which this enzyme is overexpressed polypeptides, and isolated with a L- amino -N- acyltransferase activity. Substrates of this enzyme include analogs or derivatives thereof and methionine primarily. Overexpression in sulfur-containing amino acid-...
(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. Living cells of RL-25 also displayed a 5-fold decreased rate of lipid A biosynthesis at the nonpermissive temperature as judged by a 32Pi incorporation assay. In order to examine N-acyltransferase activity directly, the substrate [alpha-32P]...
N-acyltransferase mechanism The N-acyltransferase reaction catalysed by Lnt is proposed to take place in two steps (Fig. 9,Supplementary Fig. 9). The first is an acyl transfer reaction where the acyl chain at thesn-1 position of the substrate, preferentially PE in the case of Lnt fromE. c...
BCG_2070c is identical to an ORF with proven N-acyltransferase activity since M. tuberculosis Lnt complemented the M. smegmatis lnt deletion mutant and all three residues of the catalytic triad essential for Lnt function in E. coli are conserved. Lnt activity of BCG_2279c may be buried by ...
acyltransferase activity (Cadas, Gaillet, Beltramo, Venance, & Piomelli, 1996;Cadas, Schinelli, & Piomelli, 1996;Cadas et al., 1997), which was identified as the ɛisoformof cytosolic phospholipase A2(PLA2A4E) (Ogura, Parsons, Kamat, & Cravatt, 2016). As PLA2A4E activity is n...
2a,b). The C-terminal region of Ngs1 has a weak homology to the N-Acyltransferase (NAT) domain in the GCN5-related N-acetyltransferase (GNAT) family31,32 (Fig. 2a). In front of the NAT domain lays a putative nuclear localization signal (NLS), predicted by cNLS Mapper33 (Fig. 2a)...
The use of bisubstrate analog inhibitors in structural experiments of AANAT provided a very powerful tool to probe the subtle differences in the active site leading to either acetyltransferase or acyltransferase activity. Compound 6a was shown to have Ki values of 0.048 and 33 μmol l−1 for ...
In contrast, N-acyltransferase activity decreases during development [42]. The opposite changes of these two enzymes probably explain why the NAPE level in the ischemic brain of young rodents is much higher than that in adult rodents. NAPE-PLD−/− mice were born at the expected Mendelian ...
The isopenicillin-N acyltransferase of Penicillium chrysogenum catalyzes the conversion of the biosynthetic intermediate isopenicillin N to the hydrophobic penicillins. The isopenicillin-N acyltransferase copurified with the acyl-CoA:6-aminopenicillanic acid (6-APA) acyltransferase activity which transfers ...
产品介绍 This gene encodes a single-pass membrane protein, which contains a conserved sequence of the GCN5 or NAT superfamily of N-acetyltransferases and is a member of the N-acyltransferase (NAT) superfamily. This protein is a neuron-specific protein and is the N-acetylaspartate (NAA) biosynt...