HH-Z-253 YPet-N1(Plasmid #54637)瞬转荧光蛋白质粒513nm激发,527发射 HH-Z-252 pmKeima-Red-Mito-7 HH-Z-251 smURFP-N1瞬转荧光蛋白质粒近红外荧光蛋白质粒 HH-Z-250 pmiRFP720-N1(Plasmid #136560)瞬转荧光蛋白质粒近红外荧光蛋白质粒 HH-Z-249 miRFP670nano-pcDNA(Plasmid #127443)近红外荧光蛋白...
Plasmid constructions Human cDNAs for c-Myc, Fbxw7, ODC and NPM1 were amplified from the human brain cDNA library (Clontech) by polymerase chain reaction (PCR). Human AZ1ΔT and AZ2ΔT cDNAs were prepared as described for the mouse cDNAs17. These cDNAs were inserted into the EcoRI and...
E The construction of HK2 interfering plasmid, and validation in UBTD1 overexpressed or control HCT116 cells. F The promotion effect on lactate production and glucose uptake after UBTD1 overexpression could be diminished by HK2 knockdown in HCT116 cells. G HK2 was significantly upregulated after...
A FLAG-USP1 (WT USP1) plasmid and a plasmid FLAG-USP1 C90S (catalytic鈥搃nactive mutant) were used to overexpress USP1 in T24 cells. CCK8, colony formation, and Transwell assays were used to assess cell viability, proliferation, and migration. RNA-sequencing (RNA-seq) and dual-luciferase...