HH-Z-248 IFP2.0-N1(Plasmid #54785)瞬转荧光蛋白质粒近红外荧光蛋白质粒 HH-Z-247 BDFP1.1-N1瞬转荧光蛋白质粒近红外荧光蛋白质粒 HH-Z-246 pcDNA3.1(-)-Large T-IRES-Puro HH-Z-245 pcDNA3.1(-)-large T HH-Z-078 pcDNA3.1-EGFP HH-Z-243 pcDNA3.1-MCS-EYFP HH-Z-242 PB-TetOne-MCS-puro诱导...
The specific shRNAs against USP7 and pcDNA3.1-USP7 plasmid were synthesized by Genepharma (Shanghai, China), as reported previously [22]. PDAC samples In this study, two cohorts of PDAC samples were employed. A tissue microarray containing paired PDAC and nontumor pancreas tissues was used to...
E The construction of HK2 interfering plasmid, and validation in UBTD1 overexpressed or control HCT116 cells. F The promotion effect on lactate production and glucose uptake after UBTD1 overexpression could be diminished by HK2 knockdown in HCT116 cells. G HK2 was significantly upregulated after...
Plasmid constructions Human cDNAs for c-Myc, Fbxw7, ODC and NPM1 were amplified from the human brain cDNA library (Clontech) by polymerase chain reaction (PCR). Human AZ1ΔT and AZ2ΔT cDNAs were prepared as described for the mouse cDNAs17. These cDNAs were inserted into the EcoRI and...
To determine how ubiquitination affects Myc function, we initially performed transient reporter assays using an E box-dependent reporter plasmid derived from the prothymosin-α (PTMA) gene, a target for activation by Myc, and a reporter plasmid carrying the core P15INK4B promoter, a target for ...
A FLAG-USP1 (WT USP1) plasmid and a plasmid FLAG-USP1 C90S (catalytic鈥搃nactive mutant) were used to overexpress USP1 in T24 cells. CCK8, colony formation, and Transwell assays were used to assess cell viability, proliferation, and migration. RNA-sequencing (RNA-seq) and dual-luciferase...
The annealed sgRNA oligos were inserted into the lentiCRISPRv2 vector (Addgene plasmid 52961) [20] to generate the USP1-knockout plasmids. The vector was transfected into HEK293T cells with the pMD2.G and psPAX2 plasmids to produce lentivirus particles. After 48 h of lentiviral infection ...