The MYB protein contains a DNA-binding domain (DBD) at the N-terminal, which consists of three tandem repeat domains of approximately 50 amino acids containing tryptophan, named R1, R2 and R3; a conserved C-terminal negative regulatory domain (NRD); and a transactivation domain (TAD) located...
functional protein, containing two complete MYB DNA-binding domains (R2 and R3 repeats). ScMYB2S2, with the first MYB DNA-binding domain (R2 repeat) missing in the N-terminal amino acid sequence, might lim- ited its function on directly involved in transcriptional regulation. Similarly, two ...
The animal proto-oncogenec-mybwas the first gene identified to encode a MYB protein6. MYB proteins contain up to three 52-amino acid residue repeats, the R1, R2 and R3 MYB domains, which bind DNA in a sequence-specific manner. The c-myb protein contains three of these DNA-binding domain...
This review will focus on v-Myband its normal cellular counterpart c-Mybwith an emphasis on the biological and biochemical functions of their protein products. Both the v-Myb and c-Myb proteins are nuclear, bind directly to DNA, have short half-lives, and can regulate gene expression. As ...
7B), suggesting that the myb103 mutation should not affect its protein production, but may result in the significant loss of binding ability with down-stream DNA sites. As the single-base mutation caused 6 amino acid deletion in R3 binding motif of the myb103, we predicted the three-...
In the regulation of flavonoid biosynthesis, MYB repressors mainly exist in the DNA binding domain with two repeats of R2R3-MYB and a single repeat of R3-MYB. Figure 4. Phylogenetic relationships of conserved protein motifs in MYB transcription factor repressors related to anthocyanin biosynthesis ...
The family of avian myeloblastosis proto-oncogene (MYB) transcription factors (TFs) is one of the largest and most abundant of its kind in plants [1]. Each N terminus of MYB family proteins has a highly conserved DNA-binding domain (DBD) consisting of 1–4 incomplete tandem repeats (R)...
Binding assays were performed with a radiolabeled oligonucleotide containing a consensus MYB binding site. MYB-specific protein-DNA-complexes are labeled by black dots. In addition to using nuclear extracts from cells treated with Bcr-TMP in vivo, EMSA assays were performed by adding compounds ...
They contain a MYB domain (motif 2 plus motif 5) at the N-terminus, a central H1/H5-like domain (motif 7) and a C-terminally located coiled-coil domain (motif 6). The SMH protein may specifically interact through the MYB domain with telomeric double-stranded DNA in vitro, while the ...
A novel DNA binding protein with homology to myb oncoproteins containing only one repeat can function as a transcriptional activator. EMBO J. 1994;13(22):5383–92. Article CAS PubMed PubMed Central Google Scholar Elena B, Annamaria G, Eleonora C. Plant MYB transcription factors: their role ...