Environmental & Molecular MutagenesisBentley, K. S., Sarrif, A. M., Cimino, M. C. and Auletta, A. E. (1994) Assessing the risk of heritable gene mutation in mammals: Drosophila sex-linked recessive lethal test and tests measuring DNA damage and repair in mammalian germ cells. Environ. ...
By in vitro mutagenesis we changed the stop codon of CDK2 from TGA->GGA and then we cloned in frame the 129 bp of the p27Fs177 tail, starting at the site of the insertion, in frame with the CDK2 cDNA. All constructs were confirmed by sequencing. (PPT 54 KB) 12943_2009_631_MOE...
The R104Q encoding mutation was introduced by site-directed mutagenesis, and the entire cDNA was resequenced after introduction of the mutation to insure that no additional mutations were introduced. Myc-REST was the same construct used previously to define the Prickle-REST interaction.12, 13 ...
The first ENU mutagenesis applied to the self-fertilising mangrove killifish generated a variety of mutant phenotypes3,4. Among these, two lines R109 and R228 were sequenced and identified the mutations in the genes, noto and msgn1causing the phenotype of the shorttail/R109 and balltail/R228 ...
We conducted site-directed mutagenesis (Quikchange; Roche) to generate single and double KRAS mutants (G12V, G12D, Q61H, Y32F/Q61H, and Q61H/Y64F). pCMV5-Src (WT or K295RY527F), pCMV5-SHP2 (WT and C459S), pCGN-HA-HRAS WT, pCGN-HA-KRAS WT, and pCGN-HA-NRAS WT were ...
The ghd10 mutant was derived from an M2 population of the japonica rice cultivar Wuyunjing 7 (WYJ7) after EMS mutagenesis (After soaking for 24 hours, transfer the seeds into 1.5% (v/v) EMS solution at 28 degree environmental temperature and treat with 12 hours, then washing the seeds ...
in CaV1.3 channels has opposing effects on voltage- and Ca2+-dependent regulation Worawan B. Limpitikul, Ivy E. Dick, Manu Ben-Johny & David T. Yue† CaV1.3 channels are a major class of L-type Ca2+ channels which contribute to the rhythmicity of the heart and brain....
To achieve accurate mutagenesis of the entire DBD (residues 102–292, 573 bp long), we generated 4 sub-libraries (labeled “A,”“B,”“C,” and “D”), covering consecutive 141–144 bp fragments of the DBD flanked by wtp53-homologues regions (Figure 1A). These sub-libraries were sepa...
4.5. Transfection of HHIP Wild-Type and G516R Mutant Plasmids pcDNA3.1-FLAG-HHIP wild-type (WT) was purchased from Origene (Rockville, MD, USA), and pcDNA3.1-FLAG-HHIP-G516R mutant plasmid was generated by Site-Directed Mutagenesis Kits according to the manufacturer's protocol (Thermo ...
Development of a Dunaliella tertiolecta Strain with Increased Zeaxanthin Content Using Random Mutagenesis. Mar. Drugs 2017, 15, 189. [CrossRef] [PubMed] 24. North, H.M.; Frey, A.; Boutin, J.-P.; Sotta, B.; Marion-Poll, A. Analysis of xanthophyll cycle gene expression during the ...