cell-cell interactionsmutant p53p53 is an important tumor suppressor, and the complexities of p53 function in regulating cancer cell behaviour are well established. Many cancers lose or express mutant forms of p53, with evidence that the type of alteration affecting p53 may differentially impact ...
To uncover a novel mechanism by which mutant TP53 exhibits its gain-of-function properties, we looked for TP53R273Hinteracting proteins that contribute to cancer cell invasion. R273H mutation is one of the hotspot mutations inTP53in human cancers.8,11,12We first conducted a large-scale co-im...
most mutations occur in wtp53 DNA binding domain and lead to functional inactivation. Different single amino acid substitutions of the same residue also have different effects. p53 R175C mutant induces both cell cycle arrest and apoptosis, whereas p53 R175P mutant induces only...
Mutant TP53 interacts with other proteins to produce gain-of-function properties that contribute to cancer metastasis. However, the underlying mechanisms are still not fully understood. Using immunoprecipitation and proximity ligation assays, we evaluate
Thep53gene (generic name used here, also known asTP53in humans andTrp53in mouse) encodes the tumour suppressor protein p53 (TP53/TRP53), a transcription factor that can regulate diverse cellular processes, including apoptotic cell death, adaptation of cellular metabolism, cell cycle arrest, cel...
To determine if the mutant p53-PDGFRb signaling axis acts in human cancer cells, we analyzed PDGFRb expression levels in a panel of human pancreatic cancer cell lines. As in our model, PDGFRb mRNA levels were significantly higher in cells expressing mutant p53 compared to those in cell lines ...
National Institutes of Health; Bethesda, MD USA Keywords: miRNA, p53, mutant p53, wild-type p53, cancer, miRNA dysregulation REVIEW The tumor suppressor p53 is a sequence-specific transcription factor that activates the expression of genes involved in apoptosis, cell cycle arrest and senescence. p5...
Inhibition of Protein-Protein Interactions: Cell-Based Assays. 2017 Nov 20. In: Markossian, S., Grossman, A., Arkin, M., et al., editors. Assay Guidance Manual [Internet]. Bethesda (MD): Eli Lilly & Company and the National Center for Advancing Translational Sciences; 2004-. Available ...
The loss or mutation of p53 prevents mutant RAS induced cell cycle arrest and senescence, thereby promoting the malignant transformation of these cells [30]. However, in recent years some studies have provided evidence that senescent cells can under certain conditions also promote tumorigenesis [31,...
The expanding network of mutp53 protein interactions profoundly remodels the cancer cell transcriptome and proteome. Thus, p53 GOF mutants Cancers 2018, 10, 188; doi:10.3390/cancers10060188 www.mdpi.com/journal/cancers Cancers 2018, 10, 188 2 of 16 subvert a remarkable plethora of molecular ...